製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to SIA8D
  • アプリケーション
    適用あり: WB, ELISAmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Pig, Chimpanzee, African green monkey
  • 免疫原

    Synthetic peptide corresponding to a region within internal sequence amino acids 180 - 229 (DVGTKSDFIT MNPSVVQRAF GGFRNESDRE KFVHRLSMLN DSVLWIPAFM) of human SIA8D (NP_005659).

  • ポジティブ・コントロール
    • HepG2 cell lysate

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • バッファー
    Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • 特記事項(精製)
    ab82967 is purified by a peptide affinity chromatography method.
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab82967 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 41 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay, 1:312500.

ターゲット情報

  • 機能
    Catalyzes the polycondensation of alpha-2,8-linked sialic acid required for the synthesis of polysialic acid (PSA), which is present on the embryonic neural cell adhesion molecule (N-CAM), necessary for plasticity of neural cells.
  • 組織特異性
    Highly expressed in fetal brain, lung and kidney and in adult heart, spleen and thymus. Present to a lesser extent in adult brain, placenta, lung, large and small intestine and peripheral blood leukocytes.
  • パスウェイ
    Protein modification; protein glycosylation.
  • 配列類似性
    Belongs to the glycosyltransferase 29 family.
  • 細胞内局在
    Golgi apparatus membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 8-sialyltransferase 8D antibody
    • 8-sialyltransferase antibody
    • Alpha 2 8 sialyltransferase 8D antibody
    • Alpha-2 antibody
    • CMP N acetylneuraminate poly alpha 2 8 sialyl transferase antibody
    • CMP-N-acetylneuraminate-poly-alpha-2 antibody
    • Polysialyltransferase 1 antibody
    • Polysialyltransferase-1 antibody
    • PST antibody
    • PST1 antibody
    • SIA8D_HUMAN antibody
    • Sialyltransferase 8 alpha 2 8 polysialytransferase D antibody
    • Sialyltransferase 8D antibody
    • Sialytransferase St8Sia IV antibody
    • SIAT8-D antibody
    • SIAT8D antibody
    • ST8 alpha N acetyl neuraminide alpha 2 8 sialyltransferase 4 antibody
    • ST8Sia IV antibody
    • ST8SIA4 antibody
    • ST8SiaIV antibody
    see all

Anti-SIA8D antibody 画像

  • Anti-SIA8D antibody (ab82967) at 1 µg/ml (in 5% skim milk / PBS buffer) + HepG2 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 41 kDa
    Observed band size : 38 kDa (why is the actual band size different from the predicted?)

プロトコール

Anti-SIA8D antibody (ab82967) 使用論文

ab82967 has not yet been referenced specifically in any publications.

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There are currently no Abreviews or Questions for ab82967.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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