製品の概要

  • 製品名Anti-Shugoshin antibody
    Shugoshin 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal to Shugoshin
  • アプリケーション適用あり: WB, ICC/IF, Flow Cytmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Recombinant full length protein, corresponding to amino acids 1-293 of Human Shugoshin

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab58023 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 - 5 µg/ml.
This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.
ICC/IF 1/200. Use with paraformaldehyde fixed cells.
Flow Cyt Use 0.5µg for 106 cells. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

ターゲット情報

  • 機能Plays a central role in chromosome cohesion during mitosis by preventing premature dissociation of cohesin complex from centromeres after prophase, when most of cohesin complex dissociates from chromosomes arms. May act by preventing phosphorylation of the STAG2 subunit of cohesin complex at the centromere, ensuring cohesin persistence at centromere until cohesin cleavage by ESPL1/separase at anaphase. Essential for proper chromosome segregation during mitosis and this function requires interaction with PPP2R1A. Its phosphorylated form is necessary for chromosome congression and for the proper attachment of spindle microtubule to the kinetochore. Necessary for kinetochore localization of PLK1 and CENPF. May play a role in the tension sensing mechanism of the spindle-assembly checkpoint by regulating PLK1 kinetochore affinity. Isoform 3 plays a role in maintaining centriole cohesion involved in controlling spindle pole integrity.
  • 組織特異性Widely expressed. Highly expressed in testis. Expressed in lung, small intestine, breast, liver and placenta. Strongly overexpressed in 90% of breast cancers tested.
  • 配列類似性Belongs to the shugoshin family.
  • 発生段階Appears in prophase cells and remains present until metaphase. Strongly decreases at the onset of anaphase and completely disapears at telophase. Not present in interphase cells (at protein level).
  • ドメインThe KEN box and D-box 3 are required for its ubiquitination and degradation.
  • 翻訳後修飾Ubiquitinated and degraded during mitotic exit by APC/C-Cdh1.
    Phosphorylation by NEK2 is essential for chromosome congression in mitosis and for the proper attachment of spindle microtubule to the kinetochore. Phosphorylated by PLK1 and AUKRB.
  • 細胞内局在Nucleus. Chromosome > centromere. Chromosome > centromere > kinetochore. Cytoplasm > cytoskeleton > spindle pole. Cytoplasm > cytoskeleton > centrosome. Localizes to the inner centromere throughout prophase until metaphase and disappears at anaphase. During prometaphase, it localizes to a single focus, while at metaphase, it localizes to 2 spots corresponding to the 2 centromeres. Centromeric localization requires the presence of BUB1 and the interaction with PPP2R1A. Localizes to the inner kinetochore from prophase to early metaphase. Co-localizes with NEK2 and SS18L1 at the kinetochore. Phosphorylation by AUKRB and the presence of BUB1 are required for localization to the kinetochore. Isoform 1 primarily localizes to kinetochores during G2 phase and mitotic prophase, metaphase, and anaphase and does not appear to be associated with kinetochores during late mitosis. Isoform 3 is found at the centrosome in interphase and at spindle poles in mitosis and its spindle pole localization is PLK1 dependent. Isoform 3 does not localize to kinetochores during any stages of the cell cycle.
  • Information by UniProt
  • 参照データベース
  • 別名
    • hSgo 1 antibody
    • hSgo1 antibody
    • NY BR 85 antibody
    • Serologically defined breast cancer antigen NY BR 85 antibody
    • Serologically defined breast cancer antigen NY-BR-85 antibody
    • SGO 1 antibody
    • SGO antibody
    • SGO L1 antibody
    • SGO1 antibody
    • SGOL 1 antibody
    • SGOL1 antibody
    • SGOL1_HUMAN antibody
    • Shugoshin 1AB protein antibody
    • Shugoshin 1CD protein antibody
    • Shugoshin 1EFprotein antibody
    • Shugoshin 1GH protein antibody
    • Shugoshin 1KL protein antibody
    • Shugoshin like 1 (S. pombe) antibody
    • Shugoshin like 1 antibody
    • Shugoshin-like 1 antibody
    see all

Anti-Shugoshin antibody 画像

  • Western blot against tagged recombinant protein immunogen using ab58023 Shugoshin antibody at 1ug/ml. Predicted band size of immunogen is 58 kDa
  • ab58023 (1/200) detecting Shugoshin in assynchronous HeLa cells (green - image depicts a metaphase and an interphase cell). Cells were fixed in paraformaldehyde, permeabilised with 0.5% Triton X100/PBS and counterstained with DAPI (red). Please refer to abreview for further experimental details.

    See Abreview

  • Overlay histogram showing MCF-7 cells stained with ab58023 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab58023, 0.5µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in MCF-7 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Anti-Shugoshin antibody (ab58023) 使用論文

This product has been referenced in:
  • Murakami-Tonami Y  et al. SGO1 is involved in the DNA damage response in MYCN-amplified neuroblastoma cells. Sci Rep 6:31615 (2016). Mouse . Read more (PubMed: 27539729) »
  • Platani M  et al. Mio depletion links mTOR regulation to Aurora A and Plk1 activation at mitotic centrosomes. J Cell Biol 210:45-62 (2015). ICC/IF ; Human . Read more (PubMed: 26124292) »

See all 6 Publications for this product

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 22°C
Sample Human Cell (293T)
Specification 293T
Permeabilization Yes - 0.5% Triton X100
Fixative Paraformaldehyde
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Abcam user community

Verified customer

投稿 Jan 21 2015

Application Western blot
Loading amount 15 µg
Gel Running Conditions Reduced Denaturing (12% SDS-PAGE)
Sample Human Purified protein (Soluble fraction)
Specification Soluble fraction
Blocking step Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
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Abcam user community

Verified customer

投稿 Nov 25 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa)
Specification HeLa
Fixative Formaldehyde
Permeabilization Yes - PBS, 0.1% Triton
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 21°C
Username

Abcam user community

Verified customer

投稿 May 20 2011

Thank you for your inquiry. Unfortunately we have no data on cross-reactivity with Shugoshin-like 2. I aligned the sequences AAH17867.1 (292aa) used as the immunogen and Q562F6 for human SGOL2 (1265aa). The homology is very low for the sequences...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Various cell lines (amniocyte, HeLa, 293A, etc))
Loading amount 60 µg
Specification Various cell lines (amniocyte, HeLa, 293A, etc)
Gel Running Conditions Non-reduced Denaturing (8% gel)
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Ms. Noori Lee

Verified customer

投稿 Aug 07 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa)
Specification HeLa
Fixative Paraformaldehyde
Permeabilization Yes - 0.5% Triton X100 in PBS
Username

Dr. Kirk McManus

Verified customer

投稿 Feb 19 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"