製品の概要

  • 製品名Anti-SHP2 (phospho Y542) antibody [EP508(2)Y]
    SHP2 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EP508(2)Y] to SHP2 (phospho Y542)
  • 特異性ab62322 detects SHP2 phosphorylated at Tyrosine 542.
  • アプリケーション適用あり: WB, IP, ICCmore details
    適用なし: Flow Cyt
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    Synthetic phosphopeptide corresponding to residues surrounding Tyrosine 542 of human SHP2.

  • ポジティブ・コントロール
    • NIH/3T3 cell lysate.
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

     

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab62322 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/50000 - 1/100000. Detects a band of approximately 68 kDa (predicted molecular weight: 68 kDa).
IP 1/40.
ICC 1/100 - 1/250.
  • 追加情報Is unsuitable for Flow Cyt.
  • ターゲット情報

    • 機能Acts downstream of various receptor and cytoplasmic protein tyrosine kinases to participate in the signal transduction from the cell surface to the nucleus.
    • 組織特異性Widely expressed, with highest levels in heart, brain, and skeletal muscle.
    • 関連疾患Defects in PTPN11 are the cause of LEOPARD syndrome type 1 (LEOPARD1) [MIM:151100]. It is an autosomal dominant disorder allelic with Noonan syndrome. The acronym LEOPARD stands for lentigines, electrocardiographic conduction abnormalities, ocular hypertelorism, pulmonic stenosis, abnormalities of genitalia, retardation of growth, and deafness.
      Defects in PTPN11 are the cause of Noonan syndrome type 1 (NS1) [MIM:163950]. Noonan syndrome (NS) is a disorder characterized by dysmorphic facial features, short stature, hypertelorism, cardiac anomalies, deafness, motor delay, and a bleeding diathesis. Some patients with Noonan syndrome type 1 develop multiple giant cell lesions of the jaw or other bony or soft tissues, which are classified as pigmented villomoduolar synovitis (PVNS) when occurring in the jaw or joints. Note=Mutations in PTPN11 account for more than 50% of the cases. Rarely, NS is associated with juvenile myelomonocytic leukemia (JMML). NS1 inheritance is autosomal dominant.
      Defects in PTPN11 are a cause of juvenile myelomonocytic leukemia (JMML) [MIM:607785]. JMML is a pediatric myelodysplastic syndrome that constitutes approximately 30% of childhood cases of myelodysplastic syndrome (MDS) and 2% of leukemia. It is characterized by leukocytosis with tissue infiltration and in vitro hypersensitivity of myeloid progenitors to granulocyte-macrophage colony stimulating factor.
      Defects in PTPN11 are a cause of metachondromatosis (MC) [MIM:156250]. It is a skeletal disorder with radiologic fetarures of both multiple exostoses and Ollier disease, characterized by the presence of multiple enchondromas and osteochondroma-like lesions.
    • 配列類似性Belongs to the protein-tyrosine phosphatase family. Non-receptor class 2 subfamily.
      Contains 2 SH2 domains.
      Contains 1 tyrosine-protein phosphatase domain.
    • ドメインThe SH2 domains repress phosphatase activity. Binding of these domains to phosphotyrosine-containing proteins relieves this auto-inhibition, possibly by inducing a conformational change in the enzyme.
    • 翻訳後修飾Phosphorylated on Tyr-546 and Tyr-584 upon receptor protein tyrosine kinase activation; which creates a binding site for GRB2 and other SH2-containing proteins.
    • 細胞内局在Cytoplasm.
    • Information by UniProt
    • 参照データベース
    • 別名
      • BPTP3 antibody
      • CFC antibody
      • JMML antibody
      • METCDS antibody
      • MGC14433 antibody
      • NS1 antibody
      • OTTHUMP00000166107 antibody
      • OTTHUMP00000166108 antibody
      • Protein tyrosine phosphatase 2 antibody
      • Protein tyrosine phosphatase 2C antibody
      • Protein tyrosine phosphatase non receptor type 11 antibody
      • Protein-tyrosine phosphatase 1D antibody
      • Protein-tyrosine phosphatase 2C antibody
      • PTN11_HUMAN antibody
      • PTP-1D antibody
      • PTP-2C antibody
      • PTP1D antibody
      • PTP2C antibody
      • PTPN11 antibody
      • SAP2 antibody
      • SH-PTP2 antibody
      • SH-PTP3 antibody
      • SH2 domain containing protein tyrosine phosphatase 2 antibody
      • SHP 2 antibody
      • SHP-2 antibody
      • Shp2 antibody
      • SHPTP2 antibody
      • SHPTP3 antibody
      • Syp antibody
      • Tyrosine-protein phosphatase non-receptor type 11 antibody
      see all

    Anti-SHP2 (phospho Y542) antibody [EP508(2)Y] 画像

    • All lanes : Anti-SHP2 (phospho Y542) antibody [EP508(2)Y] (ab62322) at 1/50000 dilution

      Lane 1 : NIH/3T3 cell lysates; untreated
      Lane 2 : NIH/3T3 cell lysates; treated with PDGF

      Lysates/proteins at 10 µg per lane.

      Secondary
      goat anti-rabbit HRP conjugated, at 1/2000 dilution

      Predicted band size : 68 kDa
      Observed band size : 68 kDa

    Anti-SHP2 (phospho Y542) antibody [EP508(2)Y] (ab62322) 使用論文

    This product has been referenced in:
    • Furcht CM  et al. Diminished functional role and altered localization of SHP2 in non-small cell lung cancer cells with EGFR-activating mutations. Oncogene : (2012). Read more (PubMed: 22777356) »
    • Kapoor GS & O'Rourke DM SIRPalpha1 receptors interfere with the EGFRvIII signalosome to inhibit glioblastoma cell transformation and migration. Oncogene 29:4130-44 (2010). Human . Read more (PubMed: 20473329) »

    See all 3 Publications for this product

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