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The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of human SHP2 that contains serine 576.
Our Abpromise guarantee covers the use of ab17940 in the following tested applications.
|WB||1/1000. Detects a band of approximately 68 kDa (predicted molecular weight: 68 kDa).|
Peptide Competition and Phosphatase Treatment
Lysates prepared from Hek293 treated with PMA were resolved by SDS-PAGE on a 10% polyacrylamide gel and transferred to PVDF. Membranes were either treated with lambda (λ) phosphatase (1) or left untreated (2-7), blocked with a 3% BSA-TBST buffer for one hour at room temperature, and incubated with SHP2 [pS576] antibody for two hours at room temperature in a 3% BSA-TBST buffer, following prior incubation with: no peptide (1, 2), the non-phosphopeptide corresponding to the immunogen (3), a generic phosphoserine-containing peptide (4), the phosphopeptide corresponding to SHP2 [pS585] (5), the phosphopeptide corresponding to SHP2 [pS591] (6), or, the phosphopeptide immunogen (7). After washing, membranes were incubated with goat F(ab’)2 anti-rabbit IgG HRP conjugate and bands were detected using the Pierce SuperSignal™ method.
The data show that only the peptide corresponding to SHP2 [pS576] blocks the antibody signal. The data also show that phosphatase stripping eliminates the signal, verifying that the antibody is phospho-specific.
ab17940 has not yet been referenced specifically in any publications.