製品の概要

  • 製品名
    Anti-SHP1 (phospho Y536) antibody
    SHP1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to SHP1 (phospho Y536)
  • 特異性
    ab51171 detects endogenous levels of SHP1 only when phosphorylated at tyrosine 536.
  • アプリケーション
    適用あり: ELISA, IHC-P, WBmore details
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    Synthetic phosphopeptide derived from human SHP1 around the phosphorylation site of tyrosine 536 (S-E-YP-G-N).

  • ポジティブ・コントロール
    • Human breast carcinoma tissue and RAW264.7 cell extracts treated with EGF (200ng/ml, 5min).

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab51171 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA 1/10000.
IHC-P Use at an assay dependent concentration.
WB 1/300 - 1/1000. Predicted molecular weight: 68 kDa.

ターゲット情報

  • 機能
    Plays a key role in hematopoiesis. This PTPase activity may directly link growth factor receptors and other signaling proteins through protein-tyrosine phosphorylation. The SH2 regions may interact with other cellular components to modulate its own phosphatase activity against interacting substrates. Together with MTUS1, induces UBE2V2 expression upon angiotensin II stimulation.
  • 組織特異性
    Isoform 1 is expressed in hematopoietic cells. Isoform 2 is expressed in non-hematopoietic cells.
  • 配列類似性
    Belongs to the protein-tyrosine phosphatase family. Non-receptor class 2 subfamily.
    Contains 2 SH2 domains.
    Contains 1 tyrosine-protein phosphatase domain.
  • 翻訳後修飾
    Phosphorylated on serine and tyrosine residues.
  • 細胞内局在
    Cytoplasm. Nucleus. In neurons, translocates into the nucleus after treatment with angiotensin II.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 70Z-SHP antibody
    • EC 3.1.3.48 antibody
    • HCP antibody
    • HCPH antibody
    • Hematopoietic cell phosphatase antibody
    • Hematopoietic cell protein tyrosine phosphatase antibody
    • Hematopoietic cell protein-tyrosine phosphatase antibody
    • HPTP1C antibody
    • Protein tyrosine phosphatase 1C antibody
    • Protein tyrosine phosphatase non receptor type 6 antibody
    • Protein tyrosine phosphatase SHP1 antibody
    • Protein-tyrosine phosphatase 1C antibody
    • protein-tyrosine phosphatase SHP 1 antibody
    • Protein-tyrosine phosphatase SHP-1 antibody
    • PTN6_HUMAN antibody
    • PTP 1C antibody
    • PTP-1C antibody
    • PTP1C antibody
    • Ptpn6 antibody
    • SH PTP 1 antibody
    • SH PTP1 antibody
    • SH-PTP1 antibody
    • SHP 1 antibody
    • SHP 1L antibody
    • SHP1 antibody
    • SHP1L antibody
    • tyrosine protein phosphatase non receptor type 6 antibody
    • Tyrosine-protein phosphatase non-receptor type 6 antibody
    see all

画像

  • ab51171 at 1/50 dilution staining SHP1 in human breast carcinoma by Immunohistochemistry, Paraffin embedded tissue, in the absence or presence of the immunising peptide.


  • Predicted band size : 68 kDa


    Western blot analysis of extracts from RAW264.7 cells treated with EGF (200ng/ml, 5 minutes)

参考文献

This product has been referenced in:
  • Kang X  et al. The ITIM-containing receptor LAIR1 is essential for acute myeloid leukaemia development. Nat Cell Biol 17:665-77 (2015). WB . Read more (PubMed: 25915125) »
  • Abbonante V  et al. Discoidin domain receptor 1 protein is a novel modulator of megakaryocyte-collagen interactions. J Biol Chem 288:16738-46 (2013). Human . Read more (PubMed: 23530036) »

See all 4 Publications for this product

レビューと Q&A

Abcam has not validated the combination of species/application used in this Abreview.
Application
Flow Cytometry
Sample
Human Cell (Jurkat)
Specification
Jurkat
Preparation
Cell harvesting/tissue preparation method: human Jurkat cells treated with pervanadate (1 mM) for 30 min
Sample buffer: complete RPMI media
Fixation
Paraformaldehyde
Permeabilization
Yes - methanol
Gating Strategy
Jurkat
Username

Abcam user community

Verified customer

投稿 Feb 18 2013

Thank you foryour reply.

The buffers for these product do not contain stabilizing proteins.



Ab51171: Preservative: 0.02% Sodium Azide

Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium ...

Read More

Thank you for your reply.

Unfortunately none of these products has yet been tested in flow cytometry. While we do try and test as many products as we can in a large number of applications, it does seem as this group has not yet been though...

Read More

Thank you for your phone call. As I mentioned, because these are phospho-specific antibodies, we recommend using BSA as a blocking agent instead of milk. If this does not help to improve your results, please let me knowyour original order or PO numbera...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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