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RabMAb

Anti-SHP1 抗体 [EPR5519] (ab124942)

製品の概要

  • 製品名
    Anti-SHP1 antibody [EPR5519]
    SHP1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR5519] to SHP1
  • アプリケーション
    適用あり: WB, IHC-P, Flow Cyt, ICC/IFmore details
  • 種交差性
    交差種: Human
    非交差種: Mouse, Rat
  • 免疫原

    Synthetic peptide corresponding to N terminal residues of Human SHP1.

  • ポジティブ・コントロール
    • Human tonsil tissue; Jurkat, Raji and lymph node lysates
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab124942 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000 - 1/10000. Predicted molecular weight: 68 kDa.
IHC-P 1/1000 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cyt 1/10 - 1/100. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
ICC/IF 1/250 - 1/500.

ターゲット情報

  • 機能
    Plays a key role in hematopoiesis. This PTPase activity may directly link growth factor receptors and other signaling proteins through protein-tyrosine phosphorylation. The SH2 regions may interact with other cellular components to modulate its own phosphatase activity against interacting substrates. Together with MTUS1, induces UBE2V2 expression upon angiotensin II stimulation.
  • 組織特異性
    Isoform 1 is expressed in hematopoietic cells. Isoform 2 is expressed in non-hematopoietic cells.
  • 配列類似性
    Belongs to the protein-tyrosine phosphatase family. Non-receptor class 2 subfamily.
    Contains 2 SH2 domains.
    Contains 1 tyrosine-protein phosphatase domain.
  • 翻訳後修飾
    Phosphorylated on serine and tyrosine residues.
  • 細胞内局在
    Cytoplasm. Nucleus. In neurons, translocates into the nucleus after treatment with angiotensin II.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 70Z-SHP antibody
    • EC 3.1.3.48 antibody
    • HCP antibody
    • HCPH antibody
    • Hematopoietic cell phosphatase antibody
    • Hematopoietic cell protein tyrosine phosphatase antibody
    • Hematopoietic cell protein-tyrosine phosphatase antibody
    • HPTP1C antibody
    • Protein tyrosine phosphatase 1C antibody
    • Protein tyrosine phosphatase non receptor type 6 antibody
    • Protein tyrosine phosphatase SHP1 antibody
    • Protein-tyrosine phosphatase 1C antibody
    • protein-tyrosine phosphatase SHP 1 antibody
    • Protein-tyrosine phosphatase SHP-1 antibody
    • PTN6_HUMAN antibody
    • PTP 1C antibody
    • PTP-1C antibody
    • PTP1C antibody
    • Ptpn6 antibody
    • SH PTP 1 antibody
    • SH PTP1 antibody
    • SH-PTP1 antibody
    • SHP 1 antibody
    • SHP 1L antibody
    • SHP1 antibody
    • SHP1L antibody
    • tyrosine protein phosphatase non receptor type 6 antibody
    • Tyrosine-protein phosphatase non-receptor type 6 antibody
    see all

画像

  • All lanes : Anti-SHP1 antibody [EPR5519] (ab124942) at 1/1000 dilution

    Lane 1 : Jurkat cell lysate
    Lane 2 : Raji cell lysate
    Lane 3 : lymph node lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    standard HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size : 68 kDa
  • Immunocytochemistry/Immunofluorescence analysis of Raji (human Burkitt's lymphoma) cells labelling SHP1 with purified ab124942 at a dilution of 1/500. Cells were fixed with 4% Paraformaldehyde and permeabilised with 0.1% tritonX-100. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG (ab150077) at dilution of 1/1000 was used as the secondary antibody. Nuclei counterstained with DAPI (blue).

    Secondary Only Control: PBS was used instead of the primary antibody as the negative control.

  • ab124942, at a 1/1000 dilution, staining SHP1 in paraffin-embedded Human tonsil tissue by immunohistochemistry
  • Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD

参考文献

This product has been referenced in:
  • Hagberg N  et al. Systemic lupus erythematosus immune complexes increase the expression of SLAM family members CD319 (CRACC) and CD229 (LY-9) on plasmacytoid dendritic cells and CD319 on CD56(dim) NK cells. J Immunol 191:2989-98 (2013). Human . Read more (PubMed: 23956418) »

See 1 Publication for this product

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