製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to SHOX2
  • アプリケーション
    適用あり: WB, ELISAmore details
  • 種交差性
    交差種: Human, Zebrafish
    交差が予測される動物種: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog
  • 免疫原

    Synthetic peptide, corresponding to a region within internal sequence amino acids 179 - 228 (SEARVQVWFQNRRAKCRKQENQLHKGVLIGAASQFEACRVAPYVNVGAL R) of Human SHOX2, (NP_006875)

  • ポジティブ・コントロール
    • 721_B cell lysate

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab82918 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 35 kDa.Can be blocked with Human SHOX2 peptide (ab105429). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1/62500.

ターゲット情報

  • 機能
    May be a growth regulator and have a role in specifying neural systems involved in processing somatosensory information, as well as in face and body structure formation.
  • 組織特異性
    Expressed in heart, skeletal muscle, liver, lung, bone marrow fibroblast, pancreas and placenta.
  • 配列類似性
    Belongs to the paired homeobox family. Bicoid subfamily.
    Contains 1 homeobox DNA-binding domain.
  • 発生段階
    Expressed during cranofacial development as well as in heart.
  • 細胞内局在
    Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Homeobox protein Og12X antibody
    • OG 12 antibody
    • OG 12X antibody
    • OG12 antibody
    • OG12X antibody
    • OGI 2X antibody
    • OGI2X antibody
    • Paired related homeobox protein SHOT antibody
    • Paired-related homeobox protein SHOT antibody
    • Short stature homeobox 2 antibody
    • Short stature homeobox homolog antibody
    • Short stature homeobox protein 2 antibody
    • SHOT antibody
    • SHOX 2 antibody
    • SHOX homologous gene on chromosome 3 antibody
    • SHOX2 antibody
    • SHOX2_HUMAN antibody
    see all

画像

  • Anti-SHOX2 antibody (ab82918) at 1 µg/ml + 721_B cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 35 kDa
    Observed band size : 40 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 20 kDa. We are unsure as to the identity of these extra bands.
  • All lanes : Anti-SHOX2 antibody (ab82918) at 1 µg/ml

    Lane 1 : Marker
    Lane 2 : Zebrafish brain homogenate at 20 µg
    Lane 3 : Zebrafish heart homogenate at 20 µg
    Lane 4 : Zebrafish liver homogenate at 20 µg

    Secondary
    Goat polyclonal to Rabbit IgG – H&L – Pre-Adsorbed (HRP) at 1/6000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 35 kDa
    Observed band size : 41 kDa (why is the actual band size different from the predicted?)


    Exposure time : 5 minutes

プロトコール

参考文献

ab82918 has not yet been referenced specifically in any publications.

レビューと Q&A

Application
Western blot
Sample
Mouse Tissue lysate - whole (Heart)
Gel Running Conditions
Reduced Denaturing (12%)
Loading amount
250 µg
Specification
Heart
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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投稿 May 09 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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