製品の概要

  • 製品名
  • 製品の詳細
    Mouse monoclonal to SHOX2
  • アプリケーション
    適用あり: WB, IHC-Frmore details
  • 種交差性
    交差種: Rat, Human
  • 免疫原

    Recombinant fragment: SPELKDRKDD AKGMEDEGQT KIKQRRSRTN FTLEQLNELE RLFDETHYPD AFMREELSQR LGLSEARVQV WFQNRRAKCR KQENQLHK, corresponding to amino acids 117-205 of Human SHOX2

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab55740 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 35 kDa.
IHC-Fr 1/500.

ターゲット情報

  • 機能
    May be a growth regulator and have a role in specifying neural systems involved in processing somatosensory information, as well as in face and body structure formation.
  • 組織特異性
    Expressed in heart, skeletal muscle, liver, lung, bone marrow fibroblast, pancreas and placenta.
  • 配列類似性
    Belongs to the paired homeobox family. Bicoid subfamily.
    Contains 1 homeobox DNA-binding domain.
  • 発生段階
    Expressed during cranofacial development as well as in heart.
  • 細胞内局在
    Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Homeobox protein Og12X antibody
    • OG 12 antibody
    • OG 12X antibody
    • OG12 antibody
    • OG12X antibody
    • OGI 2X antibody
    • OGI2X antibody
    • Paired related homeobox protein SHOT antibody
    • Paired-related homeobox protein SHOT antibody
    • Short stature homeobox 2 antibody
    • Short stature homeobox homolog antibody
    • Short stature homeobox protein 2 antibody
    • SHOT antibody
    • SHOX 2 antibody
    • SHOX homologous gene on chromosome 3 antibody
    • SHOX2 antibody
    • SHOX2_HUMAN antibody
    see all

画像

  • ab55740 staining SHOX2 in mouse embryonic spinal cord tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde and blocked with 10% serum for 30 minutes at 20°C. Samples were incubated with primary antibody (1/500 in PBS + 10% horse serum + 0.1% Triton X-100 in PBS) for 16 hours at 4°C. An Alexa Fluor® 488-conjugated donkey anti-mouse IgG polyclonal (1/1000) was used as the secondary antibody.

    See Abreview



  • Predicted band size : 35 kDa
    SHOX2 antibody (ab55740) at 1ug/lane + PC-12 cell lysate at 25ug/lane.

参考文献

This product has been referenced in:

See 1 Publication for this product

レビューと Q&A

Application
Immunohistochemistry (Frozen sections)
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 20°C
Sample
Mouse Tissue sections (Mouse embryonic spinal cord (cryosections))
Specification
Mouse embryonic spinal cord (cryosections)
Permeabilization
No
Fixative
Paraformaldehyde
Username

Dr. Frederic Clotman

Verified customer

投稿 Jan 27 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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