Anti-SHMT2 抗体 (ab88664)

製品の概要

  • 製品名
  • 製品の詳細
    Mouse polyclonal to SHMT2
  • アプリケーション
    適用あり: WB, ICC/IFmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Full length Human SHMT2 protein (NP_005403.2).

  • ポジティブ・コントロール
    • Human spleen tissue lysate. Hela cell lysate. SHMT2 transfected 293T cell lysate. HeLa cells.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab88664 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 56 kDa.
ICC/IF Use a concentration of 10 µg/ml.

ターゲット情報

  • 機能
    Contributes to the de novo mitochondrial thymidylate biosynthesis pathway. Required to prevent uracil accumulation in mtDNA. Interconversion of serine and glycine. Associates with mitochondrial DNA.
  • パスウェイ
    One-carbon metabolism; tetrahydrofolate interconversion.
  • 配列類似性
    Belongs to the SHMT family.
  • 細胞内局在
    Mitochondrion. Mitochondrion matrix > mitochondrion nucleoid. Mitochondrion inner membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Epididymis secretory sperm binding protein Li 51e antibody
    • GLY A+ antibody
    • GLYA antibody
    • glycine auxotroph A, human complement for hamster antibody
    • Glycine hydroxymethyltransferase antibody
    • GLYM_HUMAN antibody
    • HEL S 51e antibody
    • mitochondrial antibody
    • Serine hydroxymethyltransferase 2 (mitochondrial) antibody
    • Serine hydroxymethyltransferase antibody
    • Serine hydroxymethyltransferase mitochondrial antibody
    • Serine methylase antibody
    • SHMT 2 antibody
    • SHMT antibody
    • SHMT2 antibody
    see all

画像

  • Anti-SHMT2 antibody (ab88664) at 1 µg/ml + human spleen tissue lysate at 50 µg

    Predicted band size : 56 kDa
    Observed band size : 56 kDa
  • Anti-SHMT2 antibody (ab88664) at 1 µg/ml + Hela cell lysate at 50 µg

    Predicted band size : 56 kDa
    Observed band size : 56 kDa
    Additional bands at : 42 kDa. We are unsure as to the identity of these extra bands.
  • All lanes : Anti-SHMT2 antibody (ab88664) at 1 µg/ml

    Lane 1 : SHMT2 transfected 293T cell lysate
    Lane 2 : Non transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.


    Predicted band size : 56 kDa
    Observed band size : 50 kDa (why is the actual band size different from the predicted?)
  • ab88664, at 10 µg/ml, staining SHMT2 in Hela cells by Immunofluorescence.

参考文献

ab88664 has not yet been referenced specifically in any publications.

レビューと Q&A

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (Jurkat)
Loading amount
20 µg
Specification
Jurkat
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 May 02 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (HeLa cells)
Loading amount
30 µg
Specification
HeLa cells
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Dr. Alfredo Castello

Verified customer

投稿 Apr 30 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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