製品の概要

  • 製品名Anti-SHIP antibody [EP378Y]
    SHIP 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EP378Y] to SHIP
  • アプリケーション適用あり: ICC/IF, WB, IHC-P, Flow Cyt, IPmore details
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human SHIP aa 1100 to the C-terminus (C terminal). The exact sequence is proprietary.

  • ポジティブ・コントロール
    • WB: KM3, Daudi and Ramos cell lysates. IHC-P: Human and mouse spleen tissues. ICC/IF: Jurkat and Raji cells. Flow Cyt: Raji cells. IP: Daudi cell lysate.
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

     

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab45142 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF 1/150.
WB 1/1000. Detects a band of approximately 140 kDa (predicted molecular weight: 133 kDa).

For unpurified use at 1/50000.

IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

See protocols (link: http://www.abcam.com/protocols/ihc-antigen-retrieval-protocol).

Flow Cyt 1/20 - 1/50.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

IP 1/20 - 1/50.

ターゲット情報

  • 機能Phosphatidylinositol (PtdIns) phosphatase that specifically hydrolyzes the 5-phosphate of phosphatidylinositol-3,4,5-trisphosphate (PtdIns(3,4,5)P3) to produce PtdIns(3,4)P2, thereby negatively regulating the PI3K (phosphoinositide 3-kinase) pathways. Acts as a negative regulator of B-cell antigen receptor signaling. Mediates signaling from the FC-gamma-RIIB receptor (FCGR2B), playing a central role in terminating signal transduction from activating immune/hematopoietic cell receptor systems. Acts as a negative regulator of myeloid cell proliferation/survival and chemotaxis, mast cell degranulation, immune cells homeostasis, integrin alpha-IIb/beta-3 signaling in platelets and JNK signaling in B-cells. Regulates proliferation of osteoclast precursors, macrophage programming, phagocytosis and activation and is required for endotoxin tolerance. Involved in the control of cell-cell junctions, CD32a signaling in neutrophils and modulation of EGF-induced phospholipase C activity. Key regulator of neutrophil migration, by governing the formation of the leading edge and polarization required for chemotaxis. Modulates FCGR3/CD16-mediated cytotoxicity in NK cells. Mediates the activin/TGF-beta-induced apoptosis through its Smad-dependent expression. May also hydrolyze PtdIns(1,3,4,5)P4, and could thus affect the levels of the higher inositol polyphosphates like InsP6.
  • 組織特異性Specifically expressed in immune and hematopoietic cells. Expressed in bone marrow and blood cells. Levels vary considerably within this compartment. Present in at least 74% of immature CD34+ cells, whereas within the more mature population of CD33+ cells, it is present in only 10% of cells. Present in the majority of T-cells, while it is present in a minority of B-cells (at protein level).
  • 配列類似性Belongs to the inositol-1,4,5-trisphosphate 5-phosphatase family.
    Contains 1 SH2 domain.
  • ドメインThe SH2 domain interacts with tyrosine phosphorylated forms of proteins such as SHC1 or PTPN11/SHP-2. It competes with that of GRB2 for binding to phosphorylated SHC1 to inhibit the Ras pathway. It is also required for tyrosine phosphorylation.
    The NPXY sequence motif found in many tyrosine-phosphorylated proteins is required for the specific binding of the PID domain.
  • 翻訳後修飾Tyrosine phosphorylated by the members of the SRC family after exposure to a diverse array of extracellular stimuli such as cytokines, growth factors, antibodies, chemokines, integrin ligands and hypertonic and oxidative stress. Phosphorylated upon IgG receptor FCGR2B-binding.
  • 細胞内局在Cytoplasm. Membrane. Translocates to the plasma membrane when activated, translocation is probably due to different mechanisms depending on the stimulus and cell type. Partly translocated via its SH2 domain which mediates interaction with tyrosine phosphorylated receptors such as the FC-gamma-RIIB receptor (FCGR2B) or CD16/FCGR3. Tyrosine phosphorylation may also participate to membrane localization.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Inositol polyphosphate 5 phosphatase of 145kDa antibody
    • 4 antibody
    • 5-trisphosphate 5-phosphatase 1 antibody
    • hp51CN antibody
    • Inositol polyphosphate 5 phosphatase 145kDa antibody
    • Inositol polyphosphate 5 phosphatase antibody
    • Inositol polyphosphate-5-phosphatase of 145 kDa antibody
    • INPP5D antibody
    • MGC104855 antibody
    • MGC142140 antibody
    • MGC142142 antibody
    • p150Ship antibody
    • Phosphatidylinositol 3,4,5 trisphosphate 5 phosphatase 1 antibody
    • Phosphatidylinositol-3 antibody
    • SH2 containing inositol phosphatase isoform b antibody
    • SH2 domain containing inositol 5' phosphatase 1 antibody
    • SH2 domain containing inositol phosphatase 1 antibody
    • SH2 domain-containing inositol phosphatase 1 antibody
    • SH2 domain-containing inositol-5''-phosphatase 1 antibody
    • SHIP-1 antibody
    • SHIP1 antibody
    • SHIP1_HUMAN antibody
    • Signaling inositol polyphosphate 5 phosphatase SIP 145 antibody
    • SIP-145 antibody
    • SIP145 antibody
    see all

Anti-SHIP antibody [EP378Y] 画像

  • All lanes : Anti-SHIP antibody [EP378Y] (ab45142) at 1/5000 dilution (purified)

    Lane 1 : Daudi cell lysate
    Lane 2 : Ramos cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Peroxidase-conjugated goat anti-rabbit IgG, (H+L) at 1/1000 dilution

    Predicted band size : 133 kDa
    Observed band size : 140 kDa (why is the actual band size different from the predicted?)

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Anti-SHIP antibody [EP378Y] (ab45142) at 1/1000 dilution (purified) + KM3 cell lysate at 20 µg

    Secondary
    Peroxidase-conjugated goat anti-rabbit IgG, (H+L) at 1/1000 dilution

    Predicted band size : 133 kDa
    Observed band size : 140 kDa (why is the actual band size different from the predicted?)

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse spleen tissue labelling SHIP with purified ab45142 at 1/500. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human spleen tissue labelling SHIP with purified ab45142 at 1/500. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

  • Immunocytochemistry/Immunofluorescence analysis of Raji cells labelling SHIP with purified ab45142 at 1/150. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain. ab7291, a mouse anti-tubulin (1/1000) and ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/500) were also used.

    Control 1: primary antibody (1/150) and secondary antibody, ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/500).

    Control 2: ab7291 (1/1000) and secondary antibody, ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/500).

  • Immunocytochemistry/Immunofluorescence analysis of Jurkat cells labelling SHIP with purified ab45142 at 1/150. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain. ab7291, a mouse anti-tubulin (1/1000) and ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/500) were also used.

    Control 1: primary antibody (1/150) and secondary antibody, ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/500).

    Control 2: ab7291 (1/1000) and secondary antibody, ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/500).

  • Flow Cytometry analysis of Raji cells labelling SHIP with purified ab45142 at 1/50 (red). Cells were fixed with 2% paraformaldehyde. A FITC-conjugated goat anti-rabbit IgG (1/150) was used as the secondary antibody. Black - Isotype control, rabbit monoclonal IgG. Blue - Unlabelled control, cells without incubation with primary and secondary antibodies.

  • ab45142 (purified) at 1/20 immunoprecipitating SHIP in Daudi whole cell lysate.

    Lane 1 (input): Daudi whole cell lysate (10µg)

    Lane 2 (+): ab45142 + Daudi whole cell lysate (10µg).

    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab45142 in Daudi whole cell lysate.

    For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/1500).

    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

  • Anti-SHIP antibody [EP378Y] (ab45142) at 1/50000 dilution (unpurified) + Daudi cell lysate at 10 µg

    Secondary
    HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution

    Predicted band size : 133 kDa
    Observed band size : 140 kDa (why is the actual band size different from the predicted?)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human spleen tissue labelling SHIP with unpurified ab45142 at a dilution of 1/100.

Anti-SHIP antibody [EP378Y] (ab45142) 使用論文

This product has been referenced in:
  • Honda F  et al. The kinase Btk negatively regulates the production of reactive oxygen species and stimulation-induced apoptosis in human neutrophils. Nat Immunol 13:369-78 (2012). Read more (PubMed: 22366891) »
  • McNulty S  et al. The host phosphoinositide 5-phosphatase SHIP2 regulates dissemination of vaccinia virus. J Virol 85:7402-10 (2011). WB . Read more (PubMed: 21543482) »

See all 2 Publications for this product

Product Wall

ab45142 does not share sequence homology with human SHIP2 based on BLAST result.
We don't think it would cross-react with SHIP2, but this has not been confirmed experimentally.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"