製品の概要

  • 製品名Sheep Anti-Rabbit IgG H&L (HRP)
    IgG 二次抗体 製品一覧
  • ターゲット生物種Rabbit
  • 特異性By immunoelectrophoresis and ELISA this antibody reacts specifically with Rabbit IgG and with light chains common to other Rabbit immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins.
  • アプリケーション適用あり: ICC, IHC-P, ELISA, WBmore details
  • 標識HRP

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C.
  • バッファーPreservative: 0.1% Proclin
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • 特記事項(精製)This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Horse Radish Peroxidase (HRP).
  • 特記事項(標識)Molar enzyme: antibody protein ratio is 1:1
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab97095 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC Use at an assay dependent dilution.
IHC-P 1/200 - 1/5000.
ELISA 1/10000 - 1/100000. (Primary ELISA)
WB 1/5000 - 1/50000. (Colorimetric: 1/5000-1/30000; Chemiluminescent: 1/10000-1/50000)

Sheep Anti-Rabbit IgG H&L (HRP) (ab97095) 使用論文

This product has been referenced in:
  • Tang CK  et al. The chemotherapeutic agent DMXAA as a unique IRF3-dependent type-2 vaccine adjuvant. PLoS One 8:e60038 (2013). ELISA . Read more (PubMed: 23555875) »

See 1 Publication for this product

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The secondary antibodies you mentioned might work for dot blots with the rabbit polyclonal Histone primaries, as they are guaranteed to work in Western blot which is quite similar to dot blot. However, we have not...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"