製品の概要

  • 製品名
  • 製品の詳細
    Mouse polyclonal to SHARPIN
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Full length human SHARPIN protein (AAH25244.1, 1 a.a. ~ 326 a.a)

  • ポジティブ・コントロール
    • SHARPIN transfected 293T cell lysate.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファー
    Preservative: None
    Constituents: 1X PBS, pH 7.2
  • Concentration information loading...
  • 精製度
    Protein A purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab69507 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/1000. Predicted molecular weight: 34 kDa. (predicted molecular weight: Isoform 2, 34 kDa; Isoform 1, 40 kDa).

ターゲット情報

  • 機能
    May have a role in normal immune development and control of inflammation.
  • 組織特異性
    Highly expressed in skeletal muscle and placenta and at lower levels in brain, heart, colon without mucosa, thymus, spleen, kidney, liver, small intestine, lung and peripheral blood leukocytes.
  • 配列類似性
    Contains 1 RanBP2-type zinc finger.
  • 細胞内局在
    Cytoplasm. Enriched at synaptic sites in mature neurons where it colocalizes with SHANK1.
  • Information by UniProt
  • 参照データベース
  • 別名
    • DKFZp434N1923 antibody
    • hSIPL1 antibody
    • Shank associated RH domain interacting protein antibody
    • SHANK associated RH domain interactor antibody
    • Shank interacting protein like 1 antibody
    • Shank-associated RH domain-interacting protein antibody
    • Shank-interacting protein-like 1 antibody
    • Sharpin antibody
    • SHRPN_HUMAN antibody
    • SIPL1 antibody
    see all

Anti-SHARPIN antibody 画像

  • All lanes : Anti-SHARPIN antibody (ab69507) at 1/500 dilution

    Lane 1 : SHARPIN transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 34 kDa
    Observed band size : 36 kDa (why is the actual band size different from the predicted?)

プロトコール

Anti-SHARPIN antibody (ab69507) 使用論文

This product has been referenced in:
  • Verhelst K  et al. A20 inhibits LUBAC-mediated NF-?B activation by binding linear polyubiquitin chains via its zinc finger 7. EMBO J 31:3845-55 (2012). Read more (PubMed: 23032186) »

See 1 Publication for this product

Product Wall

There are currently no Abreviews or Questions for ab69507.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

登録