Anti-SHARPIN 抗体 (ab69507)
Key features and details
- Mouse polyclonal to SHARPIN
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-SHARPIN antibody
SHARPIN 一次抗体 製品一覧 -
製品の詳細
Mouse polyclonal to SHARPIN -
由来種
Mouse -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human -
免疫原
Recombinant full length protein within Human SHARPIN aa 1-350. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: AAH25244.1 -
ポジティブ・コントロール
- SHARPIN transfected 293T cell lysate.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.40
Constituent: 100% PBS -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab69507の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/500 - 1/1000. Predicted molecular weight: 34 kDa. (predicted molecular weight: Isoform 2, 34 kDa; Isoform 1, 40 kDa).
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特記事項 |
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WB
1/500 - 1/1000. Predicted molecular weight: 34 kDa. (predicted molecular weight: Isoform 2, 34 kDa; Isoform 1, 40 kDa). |
ターゲット情報
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機能
May have a role in normal immune development and control of inflammation. -
組織特異性
Highly expressed in skeletal muscle and placenta and at lower levels in brain, heart, colon without mucosa, thymus, spleen, kidney, liver, small intestine, lung and peripheral blood leukocytes. -
配列類似性
Contains 1 RanBP2-type zinc finger. -
細胞内局在
Cytoplasm. Enriched at synaptic sites in mature neurons where it colocalizes with SHANK1. - Information by UniProt
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参照データベース
- Entrez Gene: 81858 Human
- Omim: 611885 Human
- SwissProt: Q9H0F6 Human
- Unigene: 529755 Human
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別名
- DKFZp434N1923 antibody
- hSIPL1 antibody
- Shank associated RH domain interacting protein antibody
see all
画像
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All lanes : Anti-SHARPIN antibody (ab69507) at 1/500 dilution
Lane 1 : SHARPIN transfected 293T cell lysate
Lane 2 : Non-transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
Predicted band size: 34 kDa
Observed band size: 36 kDa why is the actual band size different from the predicted?
データシートおよび資料
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Datasheet download
参考文献 (8)
ab69507 は 8 報の論文で使用されています。
- Gao J et al. Sharpin suppresses ß1-integrin activation by complexing with the ß1 tail and kindlin-1. Cell Commun Signal 17:101 (2019). PubMed: 31429758
- Dorn S et al. LUBAC and ABIN-1 Modulate TRAIL-Based NF-?B Induction in Human Embryonic Kidney 293 Cells. Biores Open Access 7:81-89 (2018). PubMed: 29862142
- Khan MH et al. The Sharpin interactome reveals a role for Sharpin in lamellipodium formation via the Arp2/3 complex. J Cell Sci 130:3094-3107 (2017). PubMed: 28775156
- Yang H et al. SHARPIN Facilitates p53 Degradation in Breast Cancer Cells. Neoplasia 19:84-92 (2017). PubMed: 28063307
- Zhuang T et al. SHARPIN stabilizes estrogen receptor a and promotes breast cancer cell proliferation. Oncotarget 8:77137-77151 (2017). PubMed: 29100376
- De Franceschi N et al. Mutually Exclusive Roles of SHARPIN in Integrin Inactivation and NF-?B Signaling. PLoS One 10:e0143423 (2015). PubMed: 26600301
- Verhelst K et al. A20 inhibits LUBAC-mediated NF-?B activation by binding linear polyubiquitin chains via its zinc finger 7. EMBO J 31:3845-55 (2012). PubMed: 23032186
- Rantala JK et al. SHARPIN is an endogenous inhibitor of ß1-integrin activation. Nat Cell Biol 13:1315-24 (2011). PubMed: 21947080