ab125188, at 6 µg/mg lysate, detecting SHARPIN in HeLa cell lysate (1 mg of immunoprecipitate, 20% loaded in the lane) by Immunoprecipitation. For the subsequent Western blot, ab125188 was used at 1 µg/ml. The blot was developed using the ECL technique, with an exposure time of 30 seconds.
Anti-SHARPIN antibody (ab125188) 使用論文
This product has been referenced in:
Yang Y et al. Essential role of the linear ubiquitin chain assembly complex in lymphoma revealed by rare germline polymorphisms. Cancer Discov4:480-93 (2014).
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