Rabbit polyclonal to SHARPIN
Horse, Cow, Pig, Chimpanzee, Ferret, Rhesus monkey, Gorilla, Orangutan, Bat
Synthetic peptide, corresponding to a region within amino acids 25-75 of Human SHARPIN (NP_112236.3).
HeLa, Jurkat and 293T whole cell lysates
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate Note: pH 7 to 8
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Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/2000 - 1/10000. Predicted molecular weight: 40 kDa.
Use at 2-10 µg/mg of lysate.
May have a role in normal immune development and control of inflammation.
Highly expressed in skeletal muscle and placenta and at lower levels in brain, heart, colon without mucosa, thymus, spleen, kidney, liver, small intestine, lung and peripheral blood leukocytes.
Contains 1 RanBP2-type zinc finger.
Cytoplasm. Enriched at synaptic sites in mature neurons where it colocalizes with SHANK1.
Information by UniProt
Shank associated RH domain interacting protein antibody
Western blot - Anti-SHARPIN antibody (ab125188)
All lanes : Anti-SHARPIN antibody (ab125188) at 0.1 µg/ml Lane 1 : HeLa whole cell lysate at 50 µg Lane 2 : HeLa whole cell lysate at 15 µg Lane 3 : 293T whole cell lysate at 50 µg Lane 4 : Jurkat whole cell lysate at 50 µg Developed using the ECL technique. Predicted band size: 40 kDa Exposure time: 3 minutes
Immunoprecipitation - Anti-SHARPIN antibody (ab125188)
ab125188, at 6 µg/mg lysate, detecting SHARPIN in HeLa cell lysate (1 mg of immunoprecipitate, 20% loaded in the lane) by Immunoprecipitation.
For the subsequent Western blot, ab125188 was used at 1 µg/ml. The blot was developed using the ECL technique, with an exposure time of 30 seconds.
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