製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to SHARP2
  • アプリケーション
    適用あり: IHC-P, WB, IPmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • 免疫原

    Synthetic peptide corresponding to a region between residues 350 and the C terminus (residue 412) of human SHARP2 (NP_003661.1).

  • ポジティブ・コントロール
    • Whole cell lysates from HeLa and 293T cells.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab70723 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P 1/500 - 1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB 1/5000 - 1/15000. Detects a band of approximately 50 kDa (predicted molecular weight: 45 kDa).
IP Use at 1-4 µg/mg of lysate.

ターゲット情報

  • 関連性
    SHARP2 (enhancer–of-split and hairy-related protein, differentially expressed in chondrocytes protein 1, DEC1) is a class B basic helix-loop-helix protein. Function: May function as a transcriptional factor for neuronal differentiation. PTM: Ubiquitinated; which may lead to proteasomal degradation
  • 細胞内局在
    Nuclear
  • 参照データベース
  • 別名
    • BHLHB2 antibody
    • bHLHe40 antibody
    • Class B Basic Helix Loop Helix Protein 2 antibody
    • Class E basic helix loop helix protein 40 antibody
    • Clast5 antibody
    • DEC1 antibody
    • Differentially expressed in chondrocytes protein 1 antibody
    • E47 interaction protein 1 antibody
    • EIP1 antibody
    • Enhancer of split and hairy related protein 2 antibody
    • SHARP 2 antibody
    • Stimulated by retinoic acid gene 13 protein antibody
    • STRA13 antibody
    see all

画像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling SHARP2 with ab70723 at 1/1000 (1µg/ml). Detection: DAB.
  • Anti-SHARP2 antibody (ab70723) at 0.1 µg/ml + Whole cell lysate from HeLa cells at 50 µg
    Developed using the ECL technique

    Predicted band size : 45 kDa
    Observed band size : 50 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 65 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 3 minutes
  • Detection of Human SHARP2 by Immunoprecipitation in whole cell lysate from 293T cells (1 mg for IP, 20% of IP loaded), using ab70723 at 3 µg/mg lysate (Lane 1). Lane 2 represents rabbit IgG IP control. Subsequent Western blot detection of SHARP2 was performed using ab70723 at 1 µg/ml. Chemiluminescence with exposure time of 30 seconds

参考文献

ab70723 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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