製品の概要

  • 製品名Anti-SHANK2 antibody [S23B-49]
    SHANK2 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [S23B-49] to SHANK2
  • 特異性Recognizes SHANK1, SHANK2, SHANK3.
  • アプリケーション適用あり: WB, IHC-P, IHC-Fr, ICC, ICC/IFmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Fusion protein, corresponding to amino acids 84-309 (SH3/PDZ domains) of Rat SHANK2 (Q9QX74).

  • ポジティブ・コントロール
    • Rat brain lysate

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファーPreservative: 0.09% Sodium Azide
    Constituents: 50% Glycerol, PBS, pH 7.4
  • Concentration information loading...
  • 精製度Protein G purified
  • ポリ/モノモノクローナル
  • クローン名S23B-49
  • アイソタイプIgG1
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab94575 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
IHC-P
IHC-Fr
ICC
ICC/IF
  • 追加情報ICC: Use at a concentration of 0.1 - 1 µg/ml.
    ICC/IF: Use at a concentration of 1 - 10 µg/ml.
    IHC-P: Use at a concentration of 0.1 - 1 µg/ml.
    IHC-Fr: Use at a concentration of 0.1 - 1 µg/ml.
    WB: Use at a concentration of 1 - 10 µg/ml. Predicted molecular weight: 159 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能Seems to be an adapter protein in the postsynaptic density (PSD) of excitatory synapses that interconnects receptors of the postsynaptic membrane including NMDA-type and metabotropic glutamate receptors, and the actin-based cytoskeleton. May play a role in the structural and functional organization of the dendritic spine and synaptic junction.
    • 組織特異性Expressed in epithelial cells (at protein level). All isoforms except isoform 7 are expressed predominantly in brain, with highest levels in olfactory bulb, cerebral cortex, cerebellum, central gray matter and hippocampus. Moderate levels of expression are seen in the caudate putamen, thalamic nuclei and brain stem. In cerebellum primarily expressed in Purkinje cells. Isoform 7 is not expressed in brain but expressed in liver, cholangiocytes and thymus. Isoform 7 is present in pancreas, colonic mucosa and thymocytes (at protein level).
    • 配列類似性Belongs to the SHANK family.
      Contains 1 PDZ (DHR) domain.
      Contains 1 SAM (sterile alpha motif) domain.
      Contains 1 SH3 domain.
    • 発生段階Expressed during early postnatal brain development, especially in the caudate putamen and thalamic nuclei. Expression in the cerebral cortex, the hippocampus and the cerebellum is moderate to high at P5 and shows a stable expression throughout development. Isoforms 1, 2, 4 and 6 are predominantly expressed in cerebellum up to the age of approximately 3 weeks. Isoform 1 expression decreases during development of cortex but slightly increases in cerebellum.
    • ドメインThe PDZ domain is required for interaction with GRID2, PLCB3, SLC9A3 and CFTR.
    • 細胞内局在Apical cell membrane. Cytoplasm. Cell junction, synapse. Cell junction, synapse, postsynaptic cell membrane, postsynaptic density. Cell projection, growth cone. Cell projection, dendritic spine. Colocalizes with cortactin in growth cones in differentiating hippocampal neurons. Present in the dendritic spines of cerebellar Purkinje cells (By similarity). Colocalizes with cortactin in growth cones in differentiating hippocampal neurons. Colocalized with PDE4D to the apical membrane of colonic crypt cells.
    • Information by UniProt
    • 参照データベース
    • 別名
      • AUTS17 antibody
      • Cortactin binding protein 1 antibody
      • Cortactin SH3 domain-binding protein antibody
      • Cortactin-binding protein 1 antibody
      • CortBP1 antibody
      • CTTNBP1 antibody
      • GKAP/SAPAP interacting protein antibody
      • GKAP/SAPAP-interacting protein antibody
      • KIAA1022 antibody
      • Proline rich synapse associated protein 1 antibody
      • Proline-rich synapse-associated protein 1 antibody
      • ProSAP1 antibody
      • SH3 and multiple ankyrin repeat domains 2 antibody
      • SH3 and multiple ankyrin repeat domains protein 2 antibody
      • SHAN2_RAT antibody
      • SHANK antibody
      • Shank2 antibody
      • SPANK-3 antibody
      see all

    Anti-SHANK2 antibody [S23B-49] 画像



    • Predicted band size : 159 kDa
      Western blot detection of SHANK2 in Rat braim membrane lysates using ab94575 at 1:1000 dilution.

    Anti-SHANK2 antibody [S23B-49] (ab94575) 使用論文

    ab94575 has not yet been referenced specifically in any publications.

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