Anti-SHANK1 抗体 [N22/21] (ab94576)
Key features and details
- Mouse monoclonal [N22/21] to SHANK1
- Suitable for: IHC-P, WB, Flow Cyt
- Reacts with: Mouse, Rat, Human
- Isotype: IgG1
製品の概要
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製品名
Anti-SHANK1 antibody [N22/21]
SHANK1 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [N22/21] to SHANK1 -
由来種
Mouse -
特異性
ab94576 does not react with SHANK2 and SHANK3 -
アプリケーション
適用あり: IHC-P, WB, Flow Cytmore details -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Fusion protein corresponding to Rat SHANK1 aa 450-700.
Database link: Q9WV48 -
ポジティブ・コントロール
- Rat brain lysate
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特記事項
The clone number has been updated from S22-21 to N22/21, both clone numbers name the same antibody clone.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
Preservative: 0.09% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
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精製度
Protein G purified -
ポリ/モノ
モノクローナル -
クローン名
N22/21 -
アイソタイプ
IgG1 -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab94576の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC-P |
Use at an assay dependent concentration.
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WB |
Use a concentration of 1 - 10 µg/ml. Predicted molecular weight: 225 kDa.
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Flow Cyt |
Use 1µg for 106 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
特記事項 |
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IHC-P
Use at an assay dependent concentration. |
WB
Use a concentration of 1 - 10 µg/ml. Predicted molecular weight: 225 kDa. |
Flow Cyt
Use 1µg for 106 cells. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
ターゲット情報
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機能
Seems to be an adapter protein in the postsynaptic density (PSD) of excitatory synapses that interconnects receptors of the postsynaptic membrane including NMDA-type and metabotropic glutamate receptors via complexes with GKAP/PSD-95 and Homer, respectively, and the actin-based cytoskeleton. Plays a role in the structural and functional organization of the dendritic spine and synaptic junction. -
組織特異性
Expressed in brain particularly in the amygdala, hippocampus, substantia nigra and thalamus. Isoform 2 seems to be expressed ubiquitously. -
配列類似性
Belongs to the SHANK family.
Contains 6 ANK repeats.
Contains 1 PDZ (DHR) domain.
Contains 1 SAM (sterile alpha motif) domain.
Contains 1 SH3 domain. -
細胞内局在
Cytoplasm. Cell junction > synapse > postsynaptic cell membrane > postsynaptic density. Cell junction > synapse. Postsynaptic density of neuronal cells. - Information by UniProt
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参照データベース
- Entrez Gene: 50944 Human
- Entrez Gene: 243961 Mouse
- Entrez Gene: 78957 Rat
- Omim: 604999 Human
- SwissProt: Q9Y566 Human
- SwissProt: D3YZU1 Mouse
- SwissProt: Q9WV48 Rat
- Unigene: 274255 Human
see all -
別名
- GKAP/SAPAP interacting protein antibody
- OTTHUMP00000174437 antibody
- SH3 and multiple ankyrin repeat domains 1 antibody
see all
画像
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Western blot detection of Shank1 in Rat brain membrane lysates using ab94576 at 1:1000 dilution.
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paraffin-embedded, bouin's-fixed mouse backskin stained for SHANK1 using ab94576 at 1/100 dilution in immunohistochemical analysis. FITC Goat Anti-Mouse (green) at 1/50 dilution was used as the secondary antibody. Filaggrinlike staining (upper layer aggregations of staining) is observed.
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Overlay histogram showing SH-SH5Y cells stained with ab94576 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab94576, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in SH-SY5Y cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (3)
ab94576 は 3 報の論文で使用されています。
- Collins SM & Galvez R Neocortical SHANK1 regulation of forebrain dependent associative learning. Neurobiol Learn Mem 155:173-179 (2018). PubMed: 30053575
- Collins SM et al. SHANK1 is differentially expressed during development in CA1 hippocampal neurons and astrocytes. Dev Neurobiol 78:363-373 (2018). PubMed: 29218848
- He Y et al. Effects of curcumin on synapses in APPswe/PS1dE9 mice. Int J Immunopathol Pharmacol 29:217-25 (2016). PubMed: 26957323