The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Predicted molecular weight: 40 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
機能Implicated in synaptic vesicle endocytosis. May recruit other proteins to membranes with high curvature.
組織特異性Brain, mostly in frontal cortex. Expressed at high level in fetal cerebellum.
配列類似性Belongs to the endophilin family. Contains 1 BAR domain. Contains 1 SH3 domain.
ドメインAn N-terminal amphipathic helix, the BAR domain and a second amphipathic helix inserted into helix 1 of the BAR domain (N-BAR domain) induce membrane curvature and bind curved membranes. The BAR domain dimer forms a rigid crescent shaped bundle of helices with the pair of second amphipathic helices protruding towards the membrane-binding surface.
細胞内局在Cytoplasm. Membrane. Concentrated in presynaptic nerve terminals in neurons.