Anti-SH2D1A/SAP 抗体 [EPR3168] (ab109120)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3168] to SH2D1A/SAP
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
製品の概要
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製品名
Anti-SH2D1A/SAP antibody [EPR3168]
SH2D1A/SAP 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR3168] to SH2D1A/SAP -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details
適用なし: Flow Cyt,ICC/IF or IHC-P -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- WB: Molt-4, Jurkat, PC-12 and K562 cell lysates and mouse brain tissue lysate.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS, 0.05% BSA -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR3168 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab109120の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/1000 - 1/10000. Detects a band of approximately 16 kDa (predicted molecular weight: 14 kDa).
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特記事項 |
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WB
1/1000 - 1/10000. Detects a band of approximately 16 kDa (predicted molecular weight: 14 kDa). |
ターゲット情報
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機能
Inhibitor of the SLAM self-association. Acts by blocking recruitment of the SH2-domain-containing signal-transduction molecule SHP-2 to a docking site in the SLAM cytoplasmic region. Mediates interaction between FYN and SLAMF1. May also regulate the activity of the neurotrophin receptors NTRK1, NTRK2 and NTRK3. -
組織特異性
Expressed at a high level in thymus and lung, with a lower level of expression in spleen and liver. Expressed in peripheral blood leukocytes, including T lymphocytes. Tends to be expressed at lower levels in peripheral blood leukocytes in patients with rheumatoid arthritis. -
関連疾患
Defects in SH2D1A are a cause of lymphoproliferative syndrome X-linked type 1 (XLP1) [MIM:308240]; also known as X-linked lymphoproliferative disease (XLPD) or Duncan disease. XLP is a rare immunodeficiency characterized by extreme susceptibility to infection with Epstein-Barr virus (EBV). Symptoms include severe or fatal mononucleosis, acquired hypogammaglobulinemia, pancytopenia and malignant lymphoma. -
配列類似性
Contains 1 SH2 domain. -
細胞内局在
Cytoplasm. - Information by UniProt
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参照データベース
- Entrez Gene: 4068 Human
- Entrez Gene: 20400 Mouse
- Entrez Gene: 501502 Rat
- Omim: 300490 Human
- SwissProt: O60880 Human
- SwissProt: O88890 Mouse
- SwissProt: B2RZ59 Rat
- Unigene: 349094 Human
see all -
別名
- DSHP antibody
- Duncan disease SH2 protein antibody
- Duncan disease SH2-protein antibody
see all
画像
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All lanes : Anti-SH2D1A/SAP antibody [EPR3168] (ab109120) at 1/2000 dilution (purified)
Lane 1 : PC-12 whole cell lysate
Lane 2 : Mouse brain tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution
Predicted band size: 14 kDa
Observed band size: 16 kDa why is the actual band size different from the predicted?Blocking and dilution buffer: 5% NFDM/TBST.
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All lanes : Anti-SH2D1A/SAP antibody [EPR3168] (ab109120) at 1/2000 dilution (purified)
Lane 1 : MOLT-4 whole cell lysate
Lane 2 : Jurkat whole cell lysate
Lane 3 : K562 whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution
Predicted band size: 14 kDa
Observed band size: 16 kDa why is the actual band size different from the predicted?Blocking and dilution buffer: 5% NFDM/TBST.
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All lanes : Anti-SH2D1A/SAP antibody [EPR3168] (ab109120) at 1/1000 dilution (unpurified)
Lane 1 : MOLT-4 cell line lysate
Lane 2 : Jurkat cell line lysate
Lane 3 : K562 cell line lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 14 kDa
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab109120 は論文での使用が確認できていません。