Chicken polyclonal to SGTA
This antibody is specific for SGTA.
Synthetic peptide corresponding to SGTA aa 61-313 (C terminal).
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Concentration information loading...
Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/2000. Predicted molecular weight: 34 kDa.
Co-chaperone that binds directly to HSC70 and HSP70 and regulates their ATPase activity.
Belongs to the SGT family.
Contains 3 TPR repeats.
The second tetratricopeptide repeat (TPR 2) mediates the interaction with SARS-CoV accessory protein 7a.
Information by UniProt
Alpha SGT antibody
Western blot - SGTA antibody - Azide free (ab37706)
Anti-SGTA antibody (ab37706) at 1/2000 dilution + E. coli-derived fusion protein at 0.5 µg
Goat anti-IgY-HRP. at 1/1000 dilution
Predicted band size:
Observed band size:
80 kDa (
why is the actual band size different from the predicted?
A fusion protein with 6xhis-NusA, which adds 470 amino acids (around 55-60 kDa to the protein) was used in this western blot. The predicted SGTA protein is around 34 kDa. Therefore, the higher than expected band size was obtained.
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