製品の概要

  • 製品名
  • 製品の詳細
    Chicken polyclonal to SGTA
  • 由来種
    Chicken
  • 特異性
    This antibody is specific for SGTA.
  • アプリケーション
    適用あり: WB, ICC/IFmore details
  • 種交差性
    交差種: Mouse
    交差が予測される動物種: Human
  • 免疫原

    Synthetic peptide corresponding to SGTA aa 61-313 (C terminal).

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab37706 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/2000. Predicted molecular weight: 34 kDa.
ICC/IF 1/100.

ターゲット情報

  • 機能
    Co-chaperone that binds directly to HSC70 and HSP70 and regulates their ATPase activity.
  • 組織特異性
    Ubiquitous.
  • 配列類似性
    Belongs to the SGT family.
    Contains 3 TPR repeats.
  • ドメイン
    The second tetratricopeptide repeat (TPR 2) mediates the interaction with SARS-CoV accessory protein 7a.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Alpha SGT antibody
    • Alpha-SGT antibody
    • AlphaSGT antibody
    • hSGT antibody
    • SGT antibody
    • Sgt1 antibody
    • SGTA antibody
    • SGTA_HUMAN antibody
    • Small glutamine rich protein with tetratricopeptide repeats 1 antibody
    • Small glutamine rich tetratricopeptide repeat containing protein alpha antibody
    • Small glutamine rich tetratricopeptide repeat TPR containing alpha antibody
    • Small glutamine rich tetratricopeptide repeat-containing protein antibody
    • Small glutamine-rich tetratricopeptide repeat-containing protein alpha antibody
    • Stg antibody
    • UBP antibody
    • Vpu binding protein antibody
    • Vpu-binding protein antibody
    see all

画像

  • Anti-SGTA antibody (ab37706) at 1/2000 dilution + E. coli-derived fusion protein at 0.5 µg

    Secondary
    Goat anti-IgY-HRP. at 1/1000 dilution

    Predicted band size: 34 kDa
    Observed band size: 80 kDa (why is the actual band size different from the predicted?)



    A fusion protein with 6xhis-NusA, which adds 470 amino acids (around 55-60 kDa to the protein) was used in this western blot. The predicted SGTA protein is around 34 kDa. Therefore, the higher than expected band size was obtained.

参考文献

This product has been referenced in:
  • Andreyeva A  et al. CHL1 is a selective organizer of the presynaptic machinery chaperoning the SNARE complex. PLoS One 5:e12018 (2010). WB ; Mouse . Read more (PubMed: 20711454) »

See 1 Publication for this product

レビューと Q&A

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (co-culture of hippocampal neurons and astrocytes)
Specification
co-culture of hippocampal neurons and astrocytes
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.25% Triton X-100 for 5 min
Blocking step
BSA as blocking agent for 20 minute(s) · Concentration: 1% · Temperature: 20°C
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投稿 Jun 15 2007

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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