製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to SGPL1
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Horse, Macaque monkey, Gorilla, Orangutan
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 500 to the C-terminus of Human SGPL1.

  • ポジティブ・コントロール
    • This antibody gave a positive control in both Human Kidney and Liver tissue lysates as well as HepG2 and HEK293 whole cell lysates.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab105183 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 63 kDa).

ターゲット情報

  • 機能
    Cleaves phosphorylated sphingoid bases (PSBs), such as sphingosine-1-phosphate, into fatty aldehydes and phosphoethanolamine. Elevates stress-induced ceramide production and apoptosis.
  • 組織特異性
    Found in liver and kidney.
  • パスウェイ
    Lipid metabolism; sphingolipid metabolism.
  • 配列類似性
    Belongs to the group II decarboxylase family. Sphingosine-1-phosphate lyase subfamily.
  • 細胞内局在
    Endoplasmic reticulum membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • FLJ13811 antibody
    • hSPL antibody
    • KIAA1252 antibody
    • S1P antibody
    • S1PL antibody
    • SGPL 1 antibody
    • SGPL1 antibody
    • SGPL1_HUMAN antibody
    • SP lyase antibody
    • SP-lyase 1 antibody
    • Sphingosine 1 phosphate aldolase antibody
    • Sphingosine 1 phosphate lyase 1 antibody
    • Sphingosine-1-phosphate aldolase antibody
    • Sphingosine-1-phosphate lyase 1 antibody
    • SPL 1 antibody
    • SPL antibody
    see all

画像

  • All lanes : Anti-SGPL1 antibody (ab105183) at 1 µg/ml

    Lane 1 : Human liver tissue lysate - total protein (ab29889)
    Lane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 3 : Human kidney tissue lysate - total protein (ab30203)
    Lane 4 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 63 kDa
    Observed band size : 60 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 42 kDa,51 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 8 minutes

プロトコール

参考文献

This product has been referenced in:
  • Didonna A  et al. A non-synonymous single-nucleotide polymorphism associated with multiple sclerosis risk affects the EVI5 interactome. Hum Mol Genet 24:7151-8 (2015). Read more (PubMed: 26433934) »

See 1 Publication for this product

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