製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to SGLT1
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide corresponding to an area surrounding amino acid 594 of Human SGLT1.

  • ポジティブ・コントロール
    • Jurkat cell lysate

法規制情報

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab97682 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 0.5 - 4 µg/ml. Detects a band of approximately 73 kDa (predicted molecular weight: 73 kDa). A ~50 kDa cleavage fragment can also be detected in Jurkat cell lysate.

ターゲット情報

  • 機能
    Actively transports glucose into cells by Na(+) cotransport with a Na(+) to glucose coupling ratio of 2:1. Efficient substrate transport in mammalian kidney is provided by the concerted action of a low affinity high capacity and a high affinity low capacity Na(+)/glucose cotransporter arranged in series along kidney proximal tubules.
  • 組織特異性
    Expressed mainly in intestine and kidney.
  • 関連疾患
    Congenital glucose/galactose malabsorption
  • 配列類似性
    Belongs to the sodium:solute symporter (SSF) (TC 2.A.21) family.
  • 翻訳後修飾
    N-glycosylation is not necessary for the cotransporter function.
  • 細胞内局在
    Membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • D22S675 antibody
    • High affinity sodium glucose cotransporter 1 antibody
    • High affinity sodium glucose cotransporter antibody
    • High affinity sodium-glucose cotransporter antibody
    • Human Na+/glucose cotransporter 1 antibody
    • Na(+)/glucose cotransporter 1 antibody
    • Na+/glucose cotransporter 1 antibody
    • NAGT antibody
    • SC5A1_HUMAN antibody
    • SGLT 1 antibody
    • SGLT1 antibody
    • SLC5A1 antibody
    • Sodium glucose cotransporter 1 antibody
    • Sodium/glucose cotransporter 1 antibody
    • Solute carrier family 5 (sodium/glucose cotransporter) member 1 antibody
    • Solute carrier family 5 member 1 antibody
    see all

画像

  • Anti-SGLT1 antibody (ab97682) at 4 µg/ml + Jurkat cell lysate

    Predicted band size : 73 kDa

プロトコール

参考文献

ab97682 has not yet been referenced specifically in any publications.

レビューと Q&A

Application
Western blot
Loading amount
100 µg
Gel Running Conditions
Reduced Denaturing (SDS-acrylamide 10%)
Sample
Human Cell lysate - whole cell (enteroendocrine cell)
Specification
enteroendocrine cell
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 28°C
Username

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投稿 Sep 17 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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