Anti-SGLT1 抗体 (ab14685)
Key features and details
- Rabbit polyclonal to SGLT1
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG
製品の概要
-
製品名
Anti-SGLT1 antibody
SGLT1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to SGLT1 -
由来種
Rabbit -
特異性
The immunogen used for this product shares 75% homology with SGLT5. Cross-reactivity with this protein has not been confirmed experimentally.
-
アプリケーション
適用あり: IHC-Pmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide corresponding to Human SGLT1 aa 600-700.
-
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.02% Sodium azide
Constituents: Tris glycine, 0.5% BSA, HEPES, 30% Glycerol -
Concentration information loading...
-
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
-
Compatible Secondaries
-
Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab14685の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
IHC-P | (1) |
Use a concentration of 2 µg/ml.
|
特記事項 |
---|
IHC-P
Use a concentration of 2 µg/ml. |
ターゲット情報
-
機能
Actively transports glucose into cells by Na(+) cotransport with a Na(+) to glucose coupling ratio of 2:1. Efficient substrate transport in mammalian kidney is provided by the concerted action of a low affinity high capacity and a high affinity low capacity Na(+)/glucose cotransporter arranged in series along kidney proximal tubules. -
組織特異性
Expressed mainly in intestine and kidney. -
関連疾患
Congenital glucose/galactose malabsorption -
配列類似性
Belongs to the sodium:solute symporter (SSF) (TC 2.A.21) family. -
翻訳後修飾
N-glycosylation is not necessary for the cotransporter function. -
細胞内局在
Membrane. - Information by UniProt
-
参照データベース
- Entrez Gene: 6523 Human
- Omim: 182380 Human
- SwissProt: P13866 Human
- Unigene: 1964 Human
-
別名
- D22S675 antibody
- High affinity sodium glucose cotransporter 1 antibody
- High affinity sodium glucose cotransporter antibody
see all
画像
-
ab14685 (2µg/ml) staining SGLT1 in human ileum using an automated system (DAKO Autostainer Plus). Using this protocol there is staining of the apical epithelium cells of the villi.
Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (22)
ab14685 は 22 報の論文で使用されています。
- Du J et al. The expression and survival significance of sodium glucose transporters in pancreatic cancer. BMC Cancer 22:116 (2022). PubMed: 35090421
- Park SH et al. Angiotensin II-induced upregulation of SGLT1 and 2 contributes to human microparticle-stimulated endothelial senescence and dysfunction: protective effect of gliflozins. Cardiovasc Diabetol 20:65 (2021). PubMed: 33726768
- Ranea-Robles P et al. Peroxisomal L-bifunctional protein (EHHADH) deficiency causes male-specific kidney hypertrophy and proximal tubular injury in mice. Kidney360 2:1441-1454 (2021). PubMed: 34651140
- Chiba Y et al. Sodium/glucose cotransporter 2 is expressed in choroid plexus epithelial cells and ependymal cells in human and mouse brains. Neuropathology N/A:N/A (2020). PubMed: 32488949
- Chiba Y et al. Glucose, Fructose, and Urate Transporters in the Choroid Plexus Epithelium. Int J Mol Sci 21:N/A (2020). PubMed: 33008107