製品の概要

  • 製品名Anti-SGK196 antibody
    SGK196 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to SGK196
  • アプリケーション適用あり: WB, ELISAmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Rabbit, Chicken, Guinea pig, Cow, Cat, Dog
  • 免疫原

    Synthetic peptide, corresponding to a region within N terminal amino acids 72-121 (LSCEELRTEVRQLKRVGEGAVKRVFLSEWKEHKVALSQLTSLEMKDDFL H) of Human SGK196, NP_115613

  • ポジティブ・コントロール
    • 721_B cell lysate

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファーPreservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab81537 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 40 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1:312500.

ターゲット情報

  • 機能Protein O-mannose kinase that specifically mediates phosphorylation at the 6-position of an O-mannose of the trisaccharide (N-acetylgalactosamine (GalNAc)-beta-1,3-N-acetylglucosamine (GlcNAc)-beta-1,4-mannose) to generate phosphorylated O-mannosyl trisaccharide (N-acetylgalactosamine-beta-1,3-N-acetylglucosamine-beta-1,4-(phosphate-6-)mannose). Phosphorylated O-mannosyl trisaccharide is a carbohydrate structure present in alpha-dystroglycan (DAG1), which is required for binding laminin G-like domain-containing extracellular proteins with high affinity. Only shows kinase activity when the GalNAc-beta-3-GlcNAc-beta-terminus is linked to the 4-position of O-mannose, suggesting that this disaccharide serves as the substrate recognition motif.
  • 関連疾患Muscular dystrophy-dystroglycanopathy congenital with brain and eye anomalies A12
  • 配列類似性Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. STKL subfamily.
    Contains 1 protein kinase domain.
  • 細胞内局在Endoplasmic reticulum membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • FLJ23356 antibody
    • MDDGA12 antibody
    • POMK antibody
    • Probable inactive protein kinase-like protein SgK196 antibody
    • Protein kinase like protein SgK196 antibody
    • Protein kinase-like protein SgK196 antibody
    • Protein O-mannose kinase antibody
    • SG196_HUMAN antibody
    • SGK196 antibody
    • Sugen kinase 196 antibody
    see all

Anti-SGK196 antibody 画像

  • Anti-SGK196 antibody (ab81537) at 1 µg/ml (in 5% skim milk / PBS buffer) + 721_B cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 40 kDa
    Observed band size : 35 kDa (why is the actual band size different from the predicted?)

Anti-SGK196 antibody (ab81537) 使用論文

ab81537 has not yet been referenced specifically in any publications.

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