The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/1000. Detects a band of approximately 60 kDa (predicted molecular weight: 49 kDa).
Use at an assay dependent concentration.
Use a concentration of 1 - 5 µg/ml.
Protein kinase that plays an important role in cellular stress response. Activates certain potassium, sodium, and chloride channels, suggesting an involvement in the regulation of processes such as cell survival, neuronal excitability and renal sodium excretion. Sustained high levels and activity may contribute to conditions such as hypertension and diabetic nephropathy. Mediates cell survival signals, phosphorylates and negatively regulates pro-apoptotic FOXO3A. Phosphorylates NEDD4L, which leads to its inactivation and to the subsequent activation of various channels and transporters such as ENaC, KCNA3/Kv1.3 or EAAT1. Isoform 2 exhibited a greater effect on cell plasma membrane expression of ENaC and Na(+) transport than isoform 1.
Expressed in most tissues with highest levels in the pancreas, followed by placenta, kidney and lung. Isoform 2 is strongly expressed in brain and pancreas, weaker in heart, placenta, lung, liver and skeletal muscle.
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. Contains 1 AGC-kinase C-terminal domain. Contains 1 protein kinase domain.
Isoform 2 subcellular localization at the plasma membrane is mediated by the sequences within the first 120 amino acids.
Regulated by phosphorylation. Phosphoinositide 3-kinase (PI3-kinase) pathway promotes phosphorylation at Ser-422 which in turn increases the phosphorylation of Thr-256 by PDPK1. Ubiquitinated by NEDD4L; which promotes proteasomal degradation. Ubiquitinated by SYVN1 at the endoplasmic reticulum; which promotes rapid proteasomal degradation and maintains a high turnover rate in resting cells. Isoform 2 shows enhanced stability. Isoform 2 resistance to proteasomal degradation is mediated by the sequences within the first 120-amino acid.
Cell membrane and Cytoplasm. Nucleus. Endoplasmic reticulum. Nuclear, upon phosphorylation.
Serum and glucocorticoid regulated kinase antibody
Serum/glucocorticoid regulated kinase 1 antibody
Serum/glucocorticoid regulated kinase antibody
Serum/glucocorticoid-regulated kinase 1 antibody
SGK 1 antibody
Sgk1 variant i3 antibody
Western blot - Anti-SGK1 (phospho S422) antibody (ab55281)
All lanes : Anti-SGK1 (phospho S422) antibody (ab55281) at 1/500 dilution
Lane 1 : Extracts from HeLa (human epithelial cell line from cervix adenocarcinoma) cells treated with Insulin (0.01 U/ml, 15 minutes) plus immunizing phosphopeptide Lane 2 : Extracts from HeLa (human epithelial cell line from cervix adenocarcinoma) cells treated with Insulin (0.01 U/ml, 15 minutes)
Developed using the ECL technique.
Predicted band size: 49 kDa
Anti-SGK1 (phospho S422) antibody (ab55281)
Immunohistochemical analysis of SGK1 (phospho S422) expression in human breast carcinoma tissue using ab55281 at a 1/50 diluton.
Left: No phosphopeptide.
Right: Sample treated with immunizing phosphopeptide.
Immunohistochemical analysis of SGK1 (phospho S422) expression in human breast carcinoma tissue using 1/50 ab55281. Left: no phosphopeptide. Right: sample treated with immunising phosphopeptide.
ICC/IF image of ab55281 stained HeLa (human epithelial cell line from cervix adenocarcinoma) cells. The cells were fixed in 4% PFA for 10 minutes and then incubated in 1% BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1 hour to permeabilize the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab55281, 1 µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1 hour. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1 hour. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43 µM.
Fu L et al. Lactic acid bacteria-specific induction of CD4(+)Foxp3(+)T cells ameliorates shrimp tropomyosin-induced allergic response in mice via suppression of mTOR signaling. Sci Rep7:1987 (2017).
Read more (PubMed: 28512288) »
Venkatesh A et al. Activated mTORC1 promotes long-term cone survival in retinitis pigmentosa mice. J Clin Invest125:1446-58 (2015).
Read more (PubMed: 25798619) »