製品の概要

  • 製品名Anti-SFRS8 antibody
    SFRS8 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to SFRS8
  • アプリケーション適用あり: WB, IP, IHC-Pmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Rhesus monkey, Gorilla, Orangutan
  • 免疫原

    Synthetic peptide corresponding to a region between residues 500 and 550 of human SFRS8 (NP_004583.2)

  • ポジティブ・コントロール
    • HeLa whole cell lysate.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファーPreservative: 0.09% Sodium Azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • 特記事項(精製)ab72044 was affinity purified using an epitope specific to SFRS8 immobilized on solid support.
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab72044 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/2000 - 1/10000. Detects a band of approximately 150 kDa (predicted molecular weight: 105 kDa).
IP Use at 2-5 µg/mg of lysate.
IHC-P 1/200 - 1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

ターゲット情報

  • 関連性SFRS8 is a human homolog of Drosophila splicing regulatory protein. This gene autoregulates its expression by control of splicing of its first two introns. In addition, it also regulates the splicing of fibronectin and CD45 genes. Multiple alternatively spliced variants have been identified. Two alternatively spliced variants have been characterized to date.
  • 細胞内局在Nuclear
  • 参照データベース
  • 別名
    • 1190005N23Rik antibody
    • 6330437E22Rik antibody
    • AI197402 antibody
    • AW212079 antibody
    • MGC167082 antibody
    • SFRS 8 antibody
    • Sfrs8 protein antibody
    • Splicing factor arginine/serine rich 8 (suppressor of white apricot Drosophila homolog) antibody
    • Splicing factor arginine/serine rich 8 (suppressor of white apricot homolog Drosophila) antibody
    • Splicing factor arginine/serine rich 8 antibody
    • Splicing factor, arginine/serine-rich 8 antibody
    • Suppressor of white apricot protein homolog antibody
    • SWAP antibody
    see all

Anti-SFRS8 antibody 画像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling SFRS8 with ab72044 at 1/200 (1µg/ml). Detection: DAB.
  • All lanes : Anti-SFRS8 antibody (ab72044) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg


    Predicted band size : 105 kDa
    Observed band size : 150 kDa (why is the actual band size different from the predicted?)


    Exposure time : 3 minutes
  • Detection of SFRS8 by Western Blot of Immunprecipitate.
    ab72044 at 1µg/ml staining SFRS8 in HeLa whole cell lysate immunoprecipitated using ab72044 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
    Detection: Chemiluminescence with exposure time of 30 seconds.

Anti-SFRS8 antibody (ab72044) 使用論文

ab72044 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"