製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to SFRS3
  • アプリケーション
    適用あり: WB, IHC-P, ICC/IF, ELISAmore details
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    Synthetic peptide corresponding to C terminal amino acids of Human SFRS3.

  • ポジティブ・コントロール
    • MCF7 cells; HeLa and Jurkat cell extracts; Human Testis tissue.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • バッファー
    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 49% PBS, 50% Glycerol, 0.88% Sodium chloride
    Note: PBS (without Mg2+, Ca2+)
  • Concentration information loading...
  • 精製度
    Protein A purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab125124 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/1000. Predicted molecular weight: 19 kDa.
IHC-P Use a concentration of 5 µg/ml.
ICC/IF 1/100 - 1/500.
ELISA 1/5000.

ターゲット情報

  • 機能
    May be involved in RNA processing in relation with cellular proliferation and/or maturation.
  • 配列類似性
    Belongs to the splicing factor SR family.
    Contains 1 RRM (RNA recognition motif) domain.
  • 翻訳後修飾
    Extensively phosphorylated on serine residues in the RS domain.
  • 細胞内局在
    Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • arginine/serine-rich 3 antibody
    • Pre mRNA splicing factor SRP20 antibody
    • Pre-mRNA-splicing factor SRP20 antibody
    • Serine/arginine-rich splicing factor 3 antibody
    • Splicing factor antibody
    • Splicing factor arginine/serine rich 20 kD antibody
    • Splicing factor arginine/serine rich 3 antibody
    • SRp20 antibody
    • SRSF3 antibody
    • SRSF3_HUMAN antibody
    • X16 antibody
    see all

Anti-SFRS3 antibody 画像



  • Performed under reducing conditions.

    Predicted band size : 19 kDa


    Exposure time : 5 seconds

    This image is courtesy of Suypriya Sen

    See Abreview

  • ab125124, at 5ug/ml, staining SFRS3 in formalin-fixed, paraffin-embedded Human testis tissue by Immunohistochemistry.
  • All lanes : Anti-SFRS3 antibody (ab125124) at 1/500 dilution

    Lane 1 : HeLa cell extract
    Lane 2 : Jurkat cell extract
    Lane 3 : Jurkat cell extract with synthesized peptide


    Predicted band size : 19 kDa
  • ab125124 at 1/100 dilution staining SRSF3 in MCF7 cells by Immunofluorescence. The image on the right is treated with the synthesized peptide.

Anti-SFRS3 antibody (ab125124) 使用論文

This product has been referenced in:
  • Lin YJ  et al. P-coumaric acid regulates exon 12 splicing of the ATP7B gene by modulating hnRNP A1 protein expressions. Biomedicine (Taipei) 5:10 (2015). Read more (PubMed: 26048696) »
  • Ajiro M  et al. Adapted Resistance to the Knockdown Effect of shRNA-Derived Srsf3 siRNAs in Mouse Littermates. Int J Biol Sci 11:1248-56 (2015). Read more (PubMed: 26435690) »

See all 3 Publications for this product

Product Wall

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 22°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate
Sample
Human Tissue sections (Liver)
Specification
Liver
Permeabilization
No
Fixative
Formaldehyde
Username

Dr. Suypriya Sen

Verified customer

投稿 Sep 05 2014

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 22°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate
Sample
Mouse Tissue sections (liver)
Specification
liver
Permeabilization
No
Fixative
Formaldehyde
Username

Dr. Suypriya Sen

Verified customer

投稿 Aug 25 2014

Application
Western blot
Sample
Mouse Cell lysate - whole cell (Primary Hepatocytes)
Loading amount
25 µg
Specification
Primary Hepatocytes
Gel Running Conditions
Reduced Denaturing (12%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Dr. Suypriya Sen

Verified customer

投稿 May 08 2013

Thank you for your enquiry.

I can confirm you are correct, these are the same antibody. We have sourced the same antibody from two different places and given them different product codes. The sources have tested them in different application...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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