製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to SFRS2IP
  • アプリケーション
    適用あり: IHC-P, WB, IPmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Rabbit, Dog, Chimpanzee, Ferret, Rhesus monkey, Gorilla, Orangutan, Bat
  • 免疫原

    Synthetic peptide corresponding to a region between residue 1098 and the C-terminus (residue 1148) of human SFRS2IP (CAA72121.1)

  • ポジティブ・コントロール
    • HeLa whole cell lysate.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー
    Preservative: 0.09% Sodium Azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • 特記事項(精製)
    ab72425 was affinity purified using an epitope specific to SFRS2IP immobilized on solid support.
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab72425 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P 1/200 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/2000 - 1/10000. Detects a band of approximately 200 kDa (predicted molecular weight: 129 kDa).
IP Use at 2-5 µg/mg of lysate.

ターゲット情報

  • 関連性
    SFRS2IP plays a role in pre-mRNA alternative splicing by regulating spliceosome assembly.
  • 細胞内局在
    Nuclear
  • 参照データベース
  • 別名
    • 1110061H03Rik antibody
    • 2610510E10Rik antibody
    • AI462454 antibody
    • CASP11 antibody
    • CTD associated SR protein 11 antibody
    • mKIAA3013 antibody
    • Renal carcinoma antigen NY REN 40 antibody
    • SC35 interacting protein 1 antibody
    • SFRS2IP antibody
    • SIP1 antibody
    • splicing factor, arginine/serine rich 2, interacting protein antibody
    • Splicing regulatory protein 129 antibody
    • SRrp129 antibody
    see all

画像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human metastatic lymph node tissue labelling SFRS2IP with ab72425 at 1/200 (1µg/ml). Detection: DAB.
  • All lanes : Anti-SFRS2IP antibody (ab72425) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg


    Predicted band size : 129 kDa
    Observed band size : 200 kDa (why is the actual band size different from the predicted?)


    Exposure time : 30 seconds
  • Detection of SFRS2IP by Western Blot of Immunprecipitate.
    ab72425 at 0.1µg/ml staining SFRS2IP in HeLa whole cell lysate immunoprecipitated using ab72425 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
    Detection: Chemiluminescence with exposure time of 10 seconds.

参考文献

ab72425 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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