製品の概要

  • 製品名Anti-SFRS2B antibody
    SFRS2B 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to SFRS2B
  • アプリケーション適用あり: WB, ELISA, IHC-Pmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide corresponding to a region within internal sequence amino acids 180-229 (YRESRYGGSH YSSSGYSNSR YSRYHSSRSH SKSGSSTSSR SASTSKSSSA) of Human SFRS2B (NP_115285).

  • ポジティブ・コントロール
    • 721_B cell lysate.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • バッファーPreservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab83006 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 32 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

Titre using peptide based assay: 1/62500.

IHC-P Use a concentration of 4 - 8 µg/ml.

ターゲット情報

  • 機能Involved in pre-mRNA alternative splicing.
  • 組織特異性Strongly expressed in pancreas, spleen and prostate. Weakly expressed in lung, liver and thymus.
  • 配列類似性Belongs to the splicing factor SR family.
    Contains 1 RRM (RNA recognition motif) domain.
  • 細胞内局在Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • arginine/serine-rich 2B antibody
    • Pre mRNA splicing factor SRP46 antibody
    • Pre-mRNA-splicing factor SRP46 antibody
    • Serine/arginine-rich splicing factor 8 antibody
    • SFRS2B antibody
    • Splicing factor antibody
    • Splicing factor arginine serine rich 46kD antibody
    • Splicing factor arginine serine rich 2B antibody
    • Splicing factor SRp46 antibody
    • SRP46 antibody
    • SRSF8 antibody
    • SRSF8_HUMAN antibody
    see all

Anti-SFRS2B antibody 画像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human heart tissue labelling SFRS2B with ab83006 at 4.0-8.0µg/ml. Positive staining shown in cardiac cells. Magnification: 400X.
  • Anti-SFRS2B antibody (ab83006) at 1 µg/ml (in 5% skim milk / PBS buffer) + 721_B cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 32 kDa
    Observed band size : 32 kDa
    Additional bands at : 30 kDa,60 kDa,>90 kDa. We are unsure as to the identity of these extra bands.
    Gel concentration: 12%

Anti-SFRS2B antibody (ab83006) 使用論文

ab83006 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab83006.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"