製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to SFRP4
  • アプリケーション
    適用あり: WB, ICC/IFmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide derived from within residues 300 - 400 of Human SFRP4.

    (Peptide available as ab32783.)

  • ポジティブ・コントロール
    • This antibody gave a positive signal in the following whole cell lysates: Hela; MCF7.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー
    Preservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab32784 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 40 kDa).Can be blocked with Human SFRP4 peptide (ab32783).
ICC/IF Use at an assay dependent concentration. PubMed: 25036934

ターゲット情報

  • 機能
    Soluble frizzled-related proteins (sFRPS) function as modulators of Wnt signaling through direct interaction with Wnts. They have a role in regulating cell growth and differentiation in specific cell types. SFRP4 may act as a regulator of adult uterine morphology and function. Increases apoptosis during ovulation possibly through modulation of FZ1/FZ4/WNT4 signaling (By similarity). Has phosphaturic effects by specifically inhibiting sodium-dependent phosphate uptake.
  • 組織特異性
    Expressed in mesenchymal cells. Highly expressed in the stroma of proliferative endometrium. Expressed in cardiomyocytes. Shows moderate to strong expression in ovarian tumors with expression increasing as the tumor stage increases. In ovarian tumors, expression levels are inversely correlated with expression of CTNNB1 (at protein level).
  • 配列類似性
    Belongs to the secreted frizzled-related protein (sFRP) family.
    Contains 1 FZ (frizzled) domain.
    Contains 1 NTR domain.
  • ドメイン
    The FZ domain is involved in binding with Wnt ligands.
  • 細胞内局在
    Secreted. Cytoplasmic in ovarian tumor cells.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Frizzled protein antibody
    • Frizzled protein human endometrium antibody
    • FRP 4 antibody
    • FRP4 antibody
    • FrpHE antibody
    • human endometrium antibody
    • PYL antibody
    • Secreted frizzled-related protein 4 antibody
    • sFRP-4 antibody
    • Sfrp4 antibody
    • SFRP4_HUMAN antibody
    see all

画像

  • All lanes : Anti-SFRP4 antibody (ab32784) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size : 40 kDa
    Observed band size : 50 kDa (why is the actual band size different from the predicted?)

参考文献

This product has been referenced in:
  • Mori K  et al. Diabetic osteopenia by decreased ß-catenin signaling is partly induced by epigenetic derepression of sFRP-4 gene. PLoS One 9:e102797 (2014). ICC/IF ; Mouse . Read more (PubMed: 25036934) »
  • Ford CE  et al. The Wnt gatekeeper SFRP4 modulates EMT, cell migration and downstream Wnt signalling in serous ovarian cancer cells. PLoS One 8:e54362 (2013). WB . Read more (PubMed: 23326605) »

See all 2 Publications for this product

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