製品の概要

  • 製品名Anti-SFRP2 antibody
    SFRP2 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to SFRP2
  • アプリケーション適用あり: WB, ELISA, IHC-P, ICC/IFmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat
  • 免疫原

    Synthetic peptide corresponding to an internal sequence of Human SFRP2.

  • ポジティブ・コントロール
    • HuvEc, COLO or HeLa cell lysate. IF/ICC: SKNSH cell line.

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab92667 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/1000. Predicted molecular weight: 33 kDa.
ELISA 1/5000.
IHC-P Use at an assay dependent concentration.
ICC/IF Use a concentration of 5 µg/ml.

ターゲット情報

  • 機能Soluble frizzled-related proteins (sFRPS) function as modulators of Wnt signaling through direct interaction with Wnts. They have a role in regulating cell growth and differentiation in specific cell types. SFRP2 may be important for eye retinal development and for myogenesis.
  • 組織特異性Expressed in adipose tissue, heart, brain, skeletal muscle, pancreas, thymus, prostate, testis, ovary, small intestine and colon. Highest levels in adipose tissue, small intestine and colon.
  • 配列類似性Belongs to the secreted frizzled-related protein (sFRP) family.
    Contains 1 FZ (frizzled) domain.
    Contains 1 NTR domain.
  • ドメインThe FZ domain is involved in binding with Wnt ligands.
  • 細胞内局在Secreted.
  • Information by UniProt
  • 参照データベース
  • 別名
    • AI851596 antibody
    • Frizzled related protein 2 antibody
    • FRP 2 antibody
    • FRP-2 antibody
    • FRP2 antibody
    • MGC128911 antibody
    • MGC153618 antibody
    • MGC53344 antibody
    • SARP 1 antibody
    • SARP-1 antibody
    • SARP1 antibody
    • SDF 5 antibody
    • SDF5 antibody
    • Secreted apoptosis related protein 1 antibody
    • Secreted apoptosis-related protein 1 antibody
    • Secreted frizzled related protein 2 antibody
    • Secreted frizzled-related protein 2 antibody
    • sFRP 2 antibody
    • sFRP-2 antibody
    • SFRP2 antibody
    • Sfrp2 protein antibody
    • SFRP2_HUMAN antibody
    see all

Anti-SFRP2 antibody 画像

  • ICC/IF image of ab92667 stained SKNSH cells. The cells were 4% fomaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab92667, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • All lanes : Anti-SFRP2 antibody (ab92667) at 1/500 dilution

    Lane 1 : HuvEc cell lysate
    Lane 2 : COLO cell lysate
    Lane 3 : HeLa cell lysate
    Lane 4 : HeLa cell lysate with immunizing peptide at 10 µg

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 33 kDa

Anti-SFRP2 antibody (ab92667) 使用論文

This product has been referenced in:
  • West JD  et al. Identification of a common Wnt-associated genetic signature across multiple cell types in pulmonary arterial hypertension. Am J Physiol Cell Physiol 307:C415-30 (2014). Read more (PubMed: 24871858) »

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (prostate)
Specification prostate
Fixative 10% Neutral buffered formalin
Antigen retrieval step Other
Permeabilization No
Username

Dr. Darragh O' Donovan

Verified customer

投稿 Jan 10 2013

Application Western blot
Sample Human Cell lysate - whole cell (benign prostate and prostate cancer cell lines)
Loading amount 50 µg
Specification benign prostate and prostate cancer cell lines
Gel Running Conditions Reduced Denaturing (15% SDS-PAGE)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 20°C
Username

Dr. Darragh O' Donovan

Verified customer

投稿 Dec 31 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"