Anti-SFRP1 抗体 [EPR7003] (ab126613)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7003] to SFRP1
- Suitable for: WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-SFRP1 antibody [EPR7003]
SFRP1 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR7003] to SFRP1 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details
適用なし: Flow Cyt -
種交差性
交差種: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- Human heart, MDA-MB-435 and A375 lysates; Human heart muscle tissue.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Stable for 12 months at -20°C. -
解離定数(KD 値)
KD = 3.01 x 10 -11 M Learn more about KD -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR7003 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab126613の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB | (1) |
1/1000 - 1/10000. Predicted molecular weight: 35 kDa.
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IHC-P | (1) |
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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特記事項 |
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WB
1/1000 - 1/10000. Predicted molecular weight: 35 kDa. |
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
ターゲット情報
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機能
Soluble frizzled-related proteins (sFRPS) function as modulators of Wnt signaling through direct interaction with Wnts. They have a role in regulating cell growth and differentiation in specific cell types. SFRP1 decreases intracellular beta-catenin levels (By similarity). Has antiproliferative effects on vascular cells, in vitro and in vivo, and can induce, in vivo, an angiogenic response. In vascular cell cycle, delays the G1 phase and entry into the S phase (By similarity). In kidney development, inhibits tubule formation and bud growth in metanephroi (By similarity). Inhibits WNT1/WNT4-mediated TCF-dependent transcription. -
組織特異性
Widely expressed. Absent from lung, liver and peripheral blood leukocytes. Highest levels in heart and fetal kidney. Also expressed in testis, ovary, fetal brain and lung, leiomyomal cells, myometrial cells and vascular smooth muscle cells. Expressed in foreskin fibroblasts and in keratinocytes. -
配列類似性
Belongs to the secreted frizzled-related protein (sFRP) family.
Contains 1 FZ (frizzled) domain.
Contains 1 NTR domain. -
ドメイン
The FZ domain is involved in binding with Wnt ligands. -
細胞内局在
Secreted. Cell membrane or extracellular matrix-associated. Released by heparin-binding. - Information by UniProt
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参照データベース
- Entrez Gene: 6422 Human
- Omim: 604156 Human
- SwissProt: Q8N474 Human
- Unigene: 213424 Human
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別名
- Frizzled related protein 1 antibody
- FRP 1 antibody
- FRP antibody
see all
画像
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Anti-SFRP1 antibody [EPR7003] (ab126613) at 1/1000 dilution (purified) + MDA-MB-435S (Human mammary gland ductal carcinoma melanocyte) whole cell lysates at 15 µg
Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/10000 dilution
Predicted band size: 35 kDaBlocking and diluting buffer: 5% NFDM/TBST
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human testis tissue sections labeling SFRP1 with Purified ab126613 at 1:50 dilution (8 μg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.
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All lanes : Anti-SFRP1 antibody [EPR7003] (ab126613) at 1/5000 dilution (purified)
Lane 1 : Human fetal kidney lysates
Lane 2 : Human fetal heart lysates
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/10000 dilution
Predicted band size: 35 kDa
Observed band size: 36 kDa why is the actual band size different from the predicted?Blocking and diluting buffer: 5% NFDM/TBST
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All lanes : Anti-SFRP1 antibody [EPR7003] (ab126613) at 1/1000 dilution (unpurified)
Lane 1 : Human heart lysate
Lane 2 : MDA-MB-435 lysate
Lane 3 : A375 lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-rabbit HRP at 1/2000 dilution
Predicted band size: 35 kDa -
Unpurified ab126613, at 1/50 dilution, staining SFRP1 in Formalin-fixed, Paraffin-embedded Human heart muscle tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (16)
ab126613 は 16 報の論文で使用されています。
- Chen C et al. Expression of Tumor Suppressor SFRP1 Predicts Biological Behaviors and Prognosis: A Potential Target for Oral Squamous Cell Carcinoma. Biomolecules 12:N/A (2022). PubMed: 35892344
- Peng X et al. Induction of Wnt signaling antagonists and p21-activated kinase enhances cardiomyocyte proliferation during zebrafish heart regeneration. J Mol Cell Biol 13:41-58 (2021). PubMed: 33582796
- Yu X et al. lncRNA SNHG1 induced by SP1 regulates bone remodeling and angiogenesis via sponging miR-181c-5p and modulating SFRP1/Wnt signaling pathway. Mol Med 27:141 (2021). PubMed: 34732133
- Higginbotham L et al. Integrated proteomics reveals brain-based cerebrospinal fluid biomarkers in asymptomatic and symptomatic Alzheimer's disease. Sci Adv 6:N/A (2020). PubMed: 33087358
- Peng JX et al. sFRP1 exerts effects on gastric cancer cells through GSK3ß/Rac1-mediated restraint of TGFß/Smad3 signaling. Oncol Rep 41:224-234 (2019). PubMed: 30542739