製品の概要

  • 製品名Anti-SF3B2 antibody
    SF3B2 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal to SF3B2
  • アプリケーション適用あり: WB, ELISAmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Recombinant full length Human SF3B2 protein (AAH07610, 1 a.a. ~ 637 a.a) with a tag of 26 kDa.

  • ポジティブ・コントロール
    • HeLa nuclear lysate. Recombinant tagged full length human SF3B2 protein.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファーPreservative: None
    Constituents: 1X PBS, pH 7.2
  • Concentration information loading...
  • 精製度Protein A purified
  • ポリ/モノモノクローナル
  • アイソタイプIgG2a
  • 軽鎖の種類kappa
  • 研究分野

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab77154 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 98 kDa.
ELISA Use at an assay dependent dilution.

ターゲット情報

  • 関連性SF3B2 is a subunit of the splicing factor SF3B required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence (BPS) in pre-mRNA. Sequence independent binding of SF3A/SF3B complex upstream of the branch site is essential, it may anchor U2 snRNP to the pre-mRNA. SF3B2 may also be involved in the assembly of the 'E' complex. It also belongs to the minor U12-dependent spliceosome, which is involved in the splicing of rare class of nuclear pre-mRNA intron.
  • 細胞内局在Nuclear
  • 参照データベース
  • 別名
    • Pre mRNA splicing factor SF3b 145 kDa subunit antibody
    • SAP 145 antibody
    • SAP145 antibody
    • SF3b1 antibody
    • SF3B145 antibody
    • SF3b150 antibody
    • Spliceosome associated protein 145 antibody
    • Splicing factor 3b subunit 2 145kDa antibody
    • Splicing factor 3b subunit 2 antibody
    see all

Anti-SF3B2 antibody 画像

  • Anti-SF3B2 antibody (ab77154) at 1 µg/ml + Recombinant tagged full length human SF3B2 protein at 0.1 µg

    Secondary
    Goat anti-mouse IgG (H&L)-HRP conjugate at 1/5000 dilution

    Predicted band size : 98 kDa
    Observed band size : 96 kDa (why is the actual band size different from the predicted?)
  • Anti-SF3B2 antibody (ab77154) at 1 µg/ml + HeLa nuclear lysate at 25 µg

    Secondary
    Goat anti-mouse IgG (H&L)-HRP conjugate at 1/2500 dilution

    Predicted band size : 98 kDa
    Observed band size : 140 kDa (why is the actual band size different from the predicted?)

Anti-SF3B2 antibody (ab77154) 使用論文

ab77154 has not yet been referenced specifically in any publications.

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There are currently no Abreviews or Questions for ab77154.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"