製品の概要

  • 製品名Anti-SF2 antibody
    SF2 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to SF2
  • アプリケーション適用あり: ICC/IF, IP, WB, IHC-Pmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Chicken, Pig, Zebrafish
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 100 - 200 of Human SF2.

    (Peptide available as ab38811.)

  • ポジティブ・コントロール
    • This antibody gave a positive result in the following whole cell lysates: HeLa (Human epithelial carcinoma cell line) Jurkat (Human T cell lymphoblast-like cell line) A431 (Human epithelial carcinoma cell line) HEK 293 (Human embryonic kidney cell line) HepG2 (Human hepatocellular liver carcinoma cell line) MCF-7 (Human breast adenocarcinoma cell line) SHSY-5Y (Human neuroblastoma cell line) This antibody gave a positive result in IHC in the following FFPE tissue: Human normal spleen.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファーPreservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab38017 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 5 µg/ml.
IP 1/500.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 34 kDa (predicted molecular weight: 27 kDa).
IHC-P Use a concentration of 5 µg/ml.

ターゲット情報

  • 機能Plays a role in preventing exon skipping, ensuring the accuracy of splicing and regulating alternative splicing. Interacts with other spliceosomal components, via the RS domains, to form a bridge between the 5'- and 3'-splice site binding components, U1 snRNP and U2AF. Can stimulate binding of U1 snRNP to a 5'-splice site-containing pre-mRNA. Binds to purine-rich RNA sequences, either the octamer, 5'-RGAAGAAC-3' (r=A or G) or the decamers, AGGACAGAGC/AGGACGAAGC. Binds preferentially to the 5'-CGAGGCG-3' motif in vitro. Three copies of the octamer constitute a powerful splicing enhancer in vitro, the ASF/SF2 splicing enhancer (ASE) which can specifically activate ASE-dependent splicing. Isoform ASF-2 and isoform ASF-3 act as splicing repressors.
  • 配列類似性Belongs to the splicing factor SR family.
    Contains 2 RRM (RNA recognition motif) domains.
  • ドメインThe RRM 2 domain plays an important role in governing both the binding mode and the phosphorylation mechanism of the RS domain by SRPK1. RS domain and RRM 2 are uniquely positioned to initiate a highly directional (C-terminus to N-terminus) phosphorylation reaction in which the RS domain slides through an extended electronegative channel separating the docking groove of SRPK1 and the active site. RRM 2 binds toward the periphery of the active site and guides the directional phosphorylation mechanism. Both the RS domain and an RRM domain are required for nucleocytoplasmic shuttling.
  • 翻訳後修飾Phosphorylated by CLK1, CLK2, CLK3 and CLK4. Phosphorylated by SRPK1 at multiple serines in its RS domain via a directional (C-terminal to N-terminal) and a dual-track mechanism incorporating both processive phosphorylation (in which the kinase stays attached to the substrate after each round of phosphorylation) and distributive phosphorylation steps (in which the kinase and substrate dissociate after each phosphorylation event). The RS domain of SRSF1 binds to a docking groove in the large lobe of the kinase domain of SRPK1 and this induces certain structural changes in SRPK1 and/or RRM 2 domain of SRSF1, allowing RRM 2 to bind the kinase and initiate phosphorylation. The cycles continue for several phosphorylation steps in a processive manner (steps 1-8) until the last few phosphorylation steps (approximately steps 9-12). During that time, a mechanical stress induces the unfolding of the beta-4 motif in RRM 2, which then docks at the docking groove of SRPK1. This also signals RRM 2 to begin to dissociate, which facilitates SRSF1 dissociation after phosphorylation is completed.
    Arg-97 is dimethylated, probably to asymmetric dimethylarginine.
  • 細胞内局在Cytoplasm. Nucleus speckle. In nuclear speckles. Shuttles between the nucleus and the cytoplasm.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Alternative splicing factor 1 antibody
    • Alternative-splicing factor 1 antibody
    • arginine/serine-rich 1 antibody
    • ASF 1 antibody
    • ASF antibody
    • ASF-1 antibody
    • ASF1 antibody
    • FLJ53078 antibody
    • MGC5228 antibody
    • P33 subunit antibody
    • Pre mRNA splicing factor SF2 P33 subunit antibody
    • pre-mRNA-splicing factor SF2 antibody
    • Serine/arginine-rich splicing factor 1 antibody
    • SF2 antibody
    • SF2P33 antibody
    • SFRS1 antibody
    • Splicing factor 2 alternate splicing factor antibody
    • Splicing factor 2 antibody
    • Splicing factor antibody
    • Splicing factor arginine/serine rich 1 antibody
    • SR Splicing factor 1 antibody
    • SRp30a antibody
    • srsf1 antibody
    • SRSF1_HUMAN antibody
    see all

Anti-SF2 antibody 画像

  • All lanes : Anti-SF2 antibody (ab38017) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : Jurkat whole cell lysate (ab7899)
    Lane 3 : A431 whole cell lysate (ab7909)
    Lane 4 : HEK293 whole cell lysate (ab7902)
    Lane 5 : HepG2 whole cell lysate (ab7900)
    Lane 6 : MCF-7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
    Lane 7 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size : 27 kDa
    Observed band size : 34 kDa (why is the actual band size different from the predicted?)
  • ICC/IF image of ab38017 stained human HeLa cells. The cells were PFA fixed (10 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab38017, 5µg/ml) for 1h at room temperature. 1%BSA / 10% normal serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).

  • All lanes : Anti-SF2 antibody (ab38017) at 1 µg/ml

    Lane 1 : HeLa whole cell lysate
    Lane 2 : 293T whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Alexa Fluor® conjugated goat anti-rabbit antibody at 1/10000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 27 kDa
    Observed band size : 34 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 50 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 10 seconds

    This image is courtesy of an anonymous Abreview

    See Abreview

  • IHC image of SF2 staining in Human normal spleen formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab38017, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-SF2 antibody (ab38017) 使用論文

This product has been referenced in:
  • Cooper-Knock J  et al. Sequestration of multiple RNA recognition motif-containing proteins by C9orf72 repeat expansions. Brain 137:2040-51 (2014). IHC-P ; Human . Read more (PubMed: 24866055) »
  • Li SJ  et al. Splicing factor transformer-2ß (Tra2ß) regulates the expression of regulator of G protein signaling 4 (RGS4) gene and is induced by morphine. PLoS One 8:e72220 (2013). WB ; Rat . Read more (PubMed: 23977258) »

See all 4 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (fibroblast)
Permeabilization Yes - Block buffer contains 0.3% TritonX-100, 1H, RT
Specification fibroblast
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative Paraformaldehyde
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投稿 Jun 29 2016

Abreviews
Application Western blot
Sample Human Cell lysate - whole cell (lymphocytes)
Gel Running Conditions Reduced Denaturing (4-12% Bis-Tris)
Loading amount 14 µg
Specification lymphocytes
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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投稿 Apr 14 2016

Thank you for contacting us.

I am sorry to hear you are experiencing difficulties with one of our products. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly.

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (Hela cells)
Specification Hela cells
Fixative Paraformaldehyde
Permeabilization Yes - 0.2% Triton X-100
Blocking step BSA as blocking agent for 20 minute(s) · Concentration: 3% · Temperature: 25°C
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投稿 Apr 23 2008

Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Hela and 293T)
Loading amount 20 µg
Specification Hela and 293T
Gel Running Conditions Reduced Denaturing (4-12%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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投稿 Apr 17 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunoprecipitation
Sample Human Cell lysate - whole cell (293T cells)
Total protein in input 500000 cells
Specification 293T cells
Immuno-precipitation step Protein A/G
Username

Abcam user community

Verified customer

投稿 Apr 16 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"