製品の概要

  • 製品名
    Anti-Sex Hormone Binding Globulin antibody
    Sex Hormone Binding Globulin 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Sex Hormone Binding Globulin
  • アプリケーション
    適用あり: ICC/IF, WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Cow, Chimpanzee, Macaque monkey, Gorilla
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Human Sex Hormone Binding Globulin.

  • ポジティブ・コントロール
    • This antibody gave a positive signal in HepG2 as well as the following Human tissue lysates: Liver; Fetal Liver; Testis. This antibody gave a positive result when used in the following formaldehyde fixed cell lines: HepG2.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー
    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS
    Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab119436 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 43 kDa).

ターゲット情報

  • 機能
    Functions as an androgen transport protein, but may also be involved in receptor mediated processes. Each dimer binds one molecule of steroid. Specific for 5-alpha-dihydrotestosterone, testosterone, and 17-beta-estradiol. Regulates the plasma metabolic clearance rate of steroid hormones by controlling their plasma concentration.
  • 組織特異性
    Isoform 1 and isoform 2 are present in liver and testis.
  • 配列類似性
    Contains 2 laminin G-like domains.
  • 細胞内局在
    Secreted. In testis, it is synthesized by the Sertoli cells, secreted into the lumen of the seminiferous tubule and transported to the epididymis.
  • Information by UniProt
  • 参照データベース
  • 別名
    • ABP antibody
    • Androgen binding protein antibody
    • SBP antibody
    • Sex hormone-binding globulin antibody
    • Sex steroid binding protein antibody
    • Sex steroid-binding protein antibody
    • SHBG antibody
    • SHBG_HUMAN antibody
    • TeBG antibody
    • Testis specific androgen binding protein antibody
    • Testis-specific androgen-binding protein antibody
    • Testosterone binding beta globulin antibody
    • Testosterone estradiol binding globulin antibody
    • Testosterone estrogen binding globulin antibody
    • Testosterone-estradiol-binding globulin antibody
    • Testosterone-estrogen-binding globulin antibody
    see all

画像

  • ab119436 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab119436 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • All lanes : Anti-Sex Hormone Binding Globulin antibody (ab119436) at 1 µg/ml

    Lane 1 : Human liver tissue lysate - total protein (ab29889)
    Lane 2 : Lung (Human) Whole Cell Lysate - fetal normal tissue (ab30282)
    Lane 3 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 4 : Human testis tissue lysate - total protein (ab30257)
    Lane 5 : Human liver tissue lysate - total protein (ab29889) with Immunizing peptide at 1 µg/ml
    Lane 6 : Lung (Human) Whole Cell Lysate - fetal normal tissue (ab30282) with Immunizing peptide at 1 µg/ml
    Lane 7 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate with Immunizing peptide at 1 µg/ml
    Lane 8 : Human testis tissue lysate - total protein (ab30257) with Immunizing peptide at 1 µg/ml

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 43 kDa
    Observed band size : 37 kDa (why is the actual band size different from the predicted?)


    Exposure time : 4 minutesThe band observed at 37 kDa could potentially be a cleaved form of Sex Hormone Binding Globulin due to the presence of a 29 amino acid signal peptide.

参考文献

This product has been referenced in:
  • Zhao C  et al. Early second-trimester plasma protein profiling using multiplexed isobaric tandem mass tag (TMT) labeling predicts gestational diabetes mellitus. Acta Diabetol 52:1103-12 (2015). WB ; Human . Read more (PubMed: 26259496) »

See 1 Publication for this product

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