Anti-SETD7 抗体 [s4E5] - ChIP Grade (ab14820)

製品の概要

  • 製品名Anti-SETD7 antibody [s4E5] - ChIP Grade
    SETD7 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [s4E5] to SETD7 - ChIP Grade
  • 特異性This antibody has been tested against recombinant SETD7 but has not been tested against the endogenous protein yet.
  • アプリケーション適用あり: ELISA, WB, ChIP, IHC-Glut, IHC-P, Flow Cyt, ICC/IFmore details
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    Recombinant full length protein (Human).

  • ポジティブ・コントロール
    • Recombinant SET7

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab14820 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA Use at an assay dependent concentration.
WB 1/250 - 1/2000. Predicted molecular weight: 50 kDa.
ChIP Use at an assay dependent concentration. PubMed: 21859860
IHC-Glut Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.
Flow Cyt Use 1µg for 106 cells.

ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

ICC/IF Use at an assay dependent concentration.

ターゲット情報

  • 機能Histone methyltransferase that specifically monomethylates 'Lys-4' of histone H3. H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation. Plays a central role in the transcriptional activation of genes such as collagenase or insulin. Recruited by IPF1/PDX-1 to the insulin promoter, leading to activate transcription. Has also methyltransferase activity toward non-histone proteins such as p53/TP53, TAF10, and possibly TAF7 by recognizing and binding the [KR]-[STA]-K in substrate proteins. Monomethylates 'Lys-189' of TAF10, leading to increase the affinity of TAF10 for RNA polymerase II. Monomethylates 'Lys-372' of p53/TP53, stabilizing p53/TP53 and increasing p53/TP53-mediated transcriptional activation. Also able to demethylated 'Lys-372' of p53/TP53 in vitro.
  • 組織特異性Widely expressed. Expressed in pancreatic islets.
  • 配列類似性Belongs to the histone-lysine methyltransferase family. SET7 subfamily.
    Contains 3 MORN repeats.
    Contains 1 SET domain.
  • ドメインThe SET domain is necessary but not sufficient for histone methyltransferase activity.
  • 細胞内局在Nucleus. Chromosome.
  • Information by UniProt
  • 参照データベース
  • 別名
    • FLJ21193 antibody
    • H3 K4 HMTase antibody
    • H3-K4-HMTase SETD7 antibody
    • H4 lysine 4 specific antibody
    • Histone H3 K4 methyltransferase antibody
    • Histone H3 lysine 4 specific methyltransferase antibody
    • Histone H3-K4 methyltransferase SETD7 antibody
    • Histone H4 K4 methyltransferase antibody
    • Histone lysine N methyltransferase antibody
    • Histone lysine N methyltransferase H3 lysine 4 specific SET7 antibody
    • Histone-lysine N-methyltransferase SETD7 antibody
    • KIAA1717 antibody
    • KMT7 antibody
    • Lysine methyltransferase antibody
    • Lysine N-methyltransferase 7 antibody
    • OTTHUMP00000164543 antibody
    • OTTHUMP00000220049 antibody
    • SET 7 antibody
    • SET 7/9 antibody
    • SET 9 antibody
    • SET D7 antibody
    • SET domain containing (lysine methyltransferase) 7 antibody
    • SET domain containing protein 7 antibody
    • SET domain containing protein 8 antibody
    • SET domain-containing protein 7 antibody
    • SET7 antibody
    • SET7/9 antibody
    • SET9 antibody
    • Setd7 antibody
    • SETD7_HUMAN antibody
    see all

Anti-SETD7 antibody [s4E5] - ChIP Grade 画像



  • Predicted band size : 50 kDa
    Western blot analysis of recombinant SET7 using ab14820 at 1/1000. Proteins were visualised using a goat anti-mouse secondary antibody conjugated to HRP and a DAB detection system. Western blot analysis of recombinant SET7 using ab14820 at 1/1000. Proteins were visualised using a goat anti-mouse secondary antibody conjugated to HRP and a DAB detection system.
  • Immunoflouresence was carried out using mouse cells in culture. Conditions: 4% PFA fixation and dilution with 0.5% triton, 10mg/ml BSA in PBS. Dilution of ab14820 used: 1/50. Green = ab14820 detected with secondary antibody (Alexa 488). Blue = DAPI stain.
  • ab14820 at 1/25 dilution staining KMT7 / SETD7 / SET7/9 in mouse pancrease tissue by immunohistochemistry (formalin/PFA-fixed paraffin-embedded sections). Sections were paraformaldehyde fixed prior to blocking in 5% serum for 1 hour at 20°C and then incubated with ab14820 for 18 hours at 4°C. A Cy2 conjugated donkey polyclonal to mouse Ig, diluted 1/20, was used as the secondary antibody.

    See Abreview

  • Overlay histogram showing HeLa cells stained with ab14820 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab14820, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells ) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.

Anti-SETD7 antibody [s4E5] - ChIP Grade (ab14820) 使用論文

This product has been referenced in:
  • Zhang Y  et al. The transcription factor GATA1 and the histone methyltransferase SET7 interact to promote VEGF-mediated angiogenesis and tumor growth and predict clinical outcome of breast cancer. Oncotarget 7:9859-75 (2016). Read more (PubMed: 26848522) »
  • Tao Y  et al. The histone methyltransferase Set7/9 promotes myoblast differentiation and myofibril assembly. J Cell Biol 194:551-65 (2011). ChIP ; Mouse . Read more (PubMed: 21859860) »

See all 3 Publications for this product

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Thank you for your continuous interest in our product.

ab14820 has already been tested and characterized for ChIP application so our classical testing Abtrial (for new untested application or species) does not qualify for the testing discount...

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The re-testing has been finished. This antibody (ab13731: Anti-SETD7 antibody - ChIP Grade) was tested on both MCF-7 and HeLa cell lysate. Please see attached results. A sing...

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