製品の概要

  • 製品名Anti-SETD7 antibody
    SETD7 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to SETD7
  • 特異性The antibody recognises the Set9 protein in yeast extract and also recognises the recombinant his tagged protein. The band is not detected in the set9 deletion mutant.
  • アプリケーション適用あり: WBmore details
  • 種交差性
    交差種: Schizosaccharomyces pombe
  • 免疫原

    Recombinant full length protein of S. pombe SET7/SET9.

  • 特記事項Accession NP_588078 (SPCC4B3.12)


    This antibody was used to demonstrate that a previously uncharacterized SET domain protein, Set9, is responsible for H4-K20 methylation in the fission yeast Schizosaccharomyces pombe. Surprisingly, H4-K20 methylation does not have any apparent role in the regulation of gene expression or heterochromatin function. Rather, we find the modification has a role in DNA damage response. Loss of Set9 activity or mutation of H4-K20 markedly impairs cell survival after genotoxic challenge and compromises the ability of cells to maintain checkpoint mediated cell cycle arrest. Genetic experiments link Set9 to Crb2, a homolog of the mammalian checkpoint protein 53BP1, and the enzyme is required for Crb2 localization to sites of DNA damage. These results argue that H4-K20 methylation functions as a "histone mark" required for the recruitment of the checkpoint protein Crb2.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファーPreservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • 精製度IgG fraction
  • 一次抗体 備考This antibody was used to demonstrate that a previously uncharacterized SET domain protein, Set9, is responsible for H4-K20 methylation in the fission yeast Schizosaccharomyces pombe. Surprisingly, H4-K20 methylation does not have any apparent role in the regulation of gene expression or heterochromatin function. Rather, we find the modification has a role in DNA damage response. Loss of Set9 activity or mutation of H4-K20 markedly impairs cell survival after genotoxic challenge and compromises the ability of cells to maintain checkpoint mediated cell cycle arrest. Genetic experiments link Set9 to Crb2, a homolog of the mammalian checkpoint protein 53BP1, and the enzyme is required for Crb2 localization to sites of DNA damage. These results argue that H4-K20 methylation functions as a "histone mark" required for the recruitment of the checkpoint protein Crb2.
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab3826 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/1000. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa).

ターゲット情報

  • 機能Histone methyltransferase that specifically monomethylates 'Lys-4' of histone H3. H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation. Plays a central role in the transcriptional activation of genes such as collagenase or insulin. Recruited by IPF1/PDX-1 to the insulin promoter, leading to activate transcription. Has also methyltransferase activity toward non-histone proteins such as p53/TP53, TAF10, and possibly TAF7 by recognizing and binding the [KR]-[STA]-K in substrate proteins. Monomethylates 'Lys-189' of TAF10, leading to increase the affinity of TAF10 for RNA polymerase II. Monomethylates 'Lys-372' of p53/TP53, stabilizing p53/TP53 and increasing p53/TP53-mediated transcriptional activation. Also able to demethylated 'Lys-372' of p53/TP53 in vitro.
  • 組織特異性Widely expressed. Expressed in pancreatic islets.
  • 配列類似性Belongs to the histone-lysine methyltransferase family. SET7 subfamily.
    Contains 3 MORN repeats.
    Contains 1 SET domain.
  • ドメインThe SET domain is necessary but not sufficient for histone methyltransferase activity.
  • 細胞内局在Nucleus. Chromosome.
  • Information by UniProt
  • 別名
    • FLJ21193 antibody
    • H3 K4 HMTase antibody
    • H3-K4-HMTase SETD7 antibody
    • H4 lysine 4 specific antibody
    • Histone H3 K4 methyltransferase antibody
    • Histone H3 lysine 4 specific methyltransferase antibody
    • Histone H3-K4 methyltransferase SETD7 antibody
    • Histone H4 K4 methyltransferase antibody
    • Histone lysine N methyltransferase antibody
    • Histone lysine N methyltransferase H3 lysine 4 specific SET7 antibody
    • Histone-lysine N-methyltransferase SETD7 antibody
    • KIAA1717 antibody
    • KMT7 antibody
    • Lysine methyltransferase antibody
    • Lysine N-methyltransferase 7 antibody
    • OTTHUMP00000164543 antibody
    • OTTHUMP00000220049 antibody
    • SET 7 antibody
    • SET 7/9 antibody
    • SET 9 antibody
    • SET D7 antibody
    • SET domain containing (lysine methyltransferase) 7 antibody
    • SET domain containing protein 7 antibody
    • SET domain containing protein 8 antibody
    • SET domain-containing protein 7 antibody
    • SET7 antibody
    • SET7/9 antibody
    • SET9 antibody
    • Setd7 antibody
    • SETD7_HUMAN antibody
    see all

Anti-SETD7 antibody 画像



  • Predicted band size : 55 kDa


    Lysate: Fission yeast extract

    Lane 1: Wildtype
    Lane 2: Set9 deletion
    Lane 3: His - 6 - tagged Set9 (1-5 ng).
    Dilution of the antibody: 1 /1000.

    Size of the band is 55 kDa.

    Steven Sanders, University of Cambridge, UK

Anti-SETD7 antibody (ab3826) 使用論文

This product has been referenced in:
  • Wang Y  et al. Regulation of Set9-mediated H4K20 methylation by a PWWP domain protein. Mol Cell 33:428-37 (2009). WB ; Schizosaccharomyces pombe . Read more (PubMed: 19250904) »
  • Sanders SL  et al. Methylation of histone H4 lysine 20 controls recruitment of Crb2 to sites of DNA damage. Cell 119:603-14 (2004). Read more (PubMed: 15550243) »

See all 2 Publications for this product

Product Wall

There are currently no Abreviews or Questions for ab3826.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"