This antibody gave a positive signal in KMT7 / SETD7 / SET7/9 Recombinant Protein as well as both HeLa and PC12 whole cell lysates.
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pH: 7.40 Preservative: 0.02% Sodium azide Constituent: PBS Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Detects a band of approximately 49 kDa (predicted molecular weight: 41 kDa).
Histone methyltransferase that specifically monomethylates 'Lys-4' of histone H3. H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation. Plays a central role in the transcriptional activation of genes such as collagenase or insulin. Recruited by IPF1/PDX-1 to the insulin promoter, leading to activate transcription. Has also methyltransferase activity toward non-histone proteins such as p53/TP53, TAF10, and possibly TAF7 by recognizing and binding the [KR]-[STA]-K in substrate proteins. Monomethylates 'Lys-189' of TAF10, leading to increase the affinity of TAF10 for RNA polymerase II. Monomethylates 'Lys-372' of p53/TP53, stabilizing p53/TP53 and increasing p53/TP53-mediated transcriptional activation. Also able to demethylated 'Lys-372' of p53/TP53 in vitro.
Widely expressed. Expressed in pancreatic islets.
Belongs to the histone-lysine methyltransferase family. SET7 subfamily. Contains 3 MORN repeats. Contains 1 SET domain.
The SET domain is necessary but not sufficient for histone methyltransferase activity.
The predicted molecular weight of SETD7 is 41 kDa (SwissProt), however we expect to observe a banding pattern around 50 kDa. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.