製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to SETD7
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Rat, Human, Recombinant fragment
    交差が予測される動物種: Rabbit, Horse, Cow, Dog, Pig, Chimpanzee, Macaque monkey, Gorilla, Chinese hamster, Orangutan
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Human SETD7.

  • ポジティブ・コントロール
    • This antibody gave a positive signal in KMT7 / SETD7 / SET7/9 Recombinant Protein as well as both HeLa and PC12 whole cell lysates.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー
    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS
    Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab114145 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 49 kDa (predicted molecular weight: 41 kDa).

ターゲット情報

  • 機能
    Histone methyltransferase that specifically monomethylates 'Lys-4' of histone H3. H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation. Plays a central role in the transcriptional activation of genes such as collagenase or insulin. Recruited by IPF1/PDX-1 to the insulin promoter, leading to activate transcription. Has also methyltransferase activity toward non-histone proteins such as p53/TP53, TAF10, and possibly TAF7 by recognizing and binding the [KR]-[STA]-K in substrate proteins. Monomethylates 'Lys-189' of TAF10, leading to increase the affinity of TAF10 for RNA polymerase II. Monomethylates 'Lys-372' of p53/TP53, stabilizing p53/TP53 and increasing p53/TP53-mediated transcriptional activation. Also able to demethylated 'Lys-372' of p53/TP53 in vitro.
  • 組織特異性
    Widely expressed. Expressed in pancreatic islets.
  • 配列類似性
    Belongs to the histone-lysine methyltransferase family. SET7 subfamily.
    Contains 3 MORN repeats.
    Contains 1 SET domain.
  • ドメイン
    The SET domain is necessary but not sufficient for histone methyltransferase activity.
  • 細胞内局在
    Nucleus. Chromosome.
  • Information by UniProt
  • 参照データベース
  • 別名
    • FLJ21193 antibody
    • H3 K4 HMTase antibody
    • H3-K4-HMTase SETD7 antibody
    • H4 lysine 4 specific antibody
    • Histone H3 K4 methyltransferase antibody
    • Histone H3 lysine 4 specific methyltransferase antibody
    • Histone H3-K4 methyltransferase SETD7 antibody
    • Histone H4 K4 methyltransferase antibody
    • Histone lysine N methyltransferase antibody
    • Histone lysine N methyltransferase H3 lysine 4 specific SET7 antibody
    • Histone-lysine N-methyltransferase SETD7 antibody
    • KIAA1717 antibody
    • KMT7 antibody
    • Lysine methyltransferase antibody
    • Lysine N-methyltransferase 7 antibody
    • OTTHUMP00000164543 antibody
    • OTTHUMP00000220049 antibody
    • SET 7 antibody
    • SET 7/9 antibody
    • SET 9 antibody
    • SET D7 antibody
    • SET domain containing (lysine methyltransferase) 7 antibody
    • SET domain containing protein 7 antibody
    • SET domain containing protein 8 antibody
    • SET domain-containing protein 7 antibody
    • SET7 antibody
    • SET7/9 antibody
    • SET9 antibody
    • Setd7 antibody
    • SETD7_HUMAN antibody
    see all

Anti-SETD7 antibody 画像

  • All lanes : Anti-SETD7 antibody (ab114145) at 1 µg/ml

    Lane 1 : Recombinant Human SETD7 protein (ab51285)
    Lane 2 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate
    Lane 3 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 41 kDa
    Observed band size : 49 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 52 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 90 seconds

プロトコール

Anti-SETD7 antibody (ab114145) 使用論文

ab114145 has not yet been referenced specifically in any publications.

Product Wall

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Permeabilization
Yes - 0.5% TritonX100 in PBS
Specification
HeLa
Fixative
Paraformaldehyde
Username

Dr. Kirk Mcmanus

Verified customer

投稿 Aug 07 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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