Anti-SESN1 抗体 (ab67156)
Key features and details
- Mouse polyclonal to SESN1
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-SESN1 antibody
SESN1 一次抗体 製品一覧 -
製品の詳細
Mouse polyclonal to SESN1 -
由来種
Mouse -
アプリケーション
適用あり: WB, ICC/IFmore details -
種交差性
交差種: Human -
免疫原
Recombinant full length protein within Human SESN1 aa 1-600. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.40
Constituent: 100% PBS -
Concentration information loading...
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精製度
Protein G purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab67156の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/500 - 1/1000. Detects a band of approximately 70 kDa (predicted molecular weight: 57 kDa).
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ICC/IF |
Use a concentration of 10 µg/ml. Requires purification with Protein A prior to ICC/IF.
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特記事項 |
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WB
1/500 - 1/1000. Detects a band of approximately 70 kDa (predicted molecular weight: 57 kDa). |
ICC/IF
Use a concentration of 10 µg/ml. Requires purification with Protein A prior to ICC/IF. |
ターゲット情報
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機能
Involved in the reduction of peroxiredoxins. May also be regulator of cellular growth. -
組織特異性
Widely expressed. -
配列類似性
Belongs to the sestrin family. -
翻訳後修飾
Phosphorylated upon DNA damage, probably by ATM or ATR. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 27244 Human
- Omim: 606103 Human
- SwissProt: Q9Y6P5 Human
- Unigene: 591336 Human
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別名
- 1110002G11Rik antibody
- AU044290 antibody
- MGC118148 antibody
see all
画像
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All lanes : Anti-SESN1 antibody (ab67156) at 1/500 dilution
Lane 1 : transfected 293T cell lysate
Lane 2 : non transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : Goat Anti-Mouse IgG (H&L)-HRP Conjugate secondary antibody at 1/2500 dilution
Predicted band size: 57 kDa
Observed band size: 75 kDa why is the actual band size different from the predicted? -
Immunofluorescence of ab67156 at 10 µg/ml on HeLa cells
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (3)
ab67156 は 3 報の論文で使用されています。
- Chollat-Namy M et al. The pharmalogical reactivation of p53 function improves breast tumor cell lysis by granzyme B and NK cells through induction of autophagy. Cell Death Dis 10:695 (2019). PubMed: 31541080
- Pulito C et al. Metformin-induced ablation of microRNA 21-5p releases Sestrin-1 and CAB39L antitumoral activities. Cell Discov 3:17022 (2017). WB ; Human . PubMed: 28698800
- Pujals A et al. Constitutive autophagy contributes to resistance to TP53-mediated apoptosis in Epstein-Barr virus-positive latency III B-cell lymphoproliferations. Autophagy 11:2275-87 (2015). PubMed: 26565591