Anti-Seryl-tRNA synthetase 抗体 (ab50146)

製品の概要

  • 製品名Anti-Seryl-tRNA synthetase antibody
    Seryl-tRNA synthetase 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Seryl-tRNA synthetase
  • 特異性This antibody reacts specifically with 60kDa SRS protein.
  • アプリケーション適用あり: WB, IPmore details
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    Recombinant full length protein (Human)

  • ポジティブ・コントロール
    • HEK293 cell lysate.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファーPreservative: 0.05% Sodium Azide
    Constituents: Whole serum
  • 精製度Whole antiserum
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab50146 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/8000 - 1/10000. Detects a band of approximately 60 kDa (predicted molecular weight: 59 kDa).
IP Use at an assay dependent dilution.

ターゲット情報

  • 機能Catalyzes the attachment of serine to tRNA(Ser). Is also probably able to aminoacylate tRNA(Sec) with serine, to form the misacylated tRNA L-seryl-tRNA(Sec), which will be further converted into selenocysteinyl-tRNA(Sec).
  • パスウェイAminoacyl-tRNA biosynthesis; selenocysteinyl-tRNA(Sec) biosynthesis; L-seryl-tRNA(Sec) from L-serine and tRNA(Sec): step 1/1.
  • 配列類似性Belongs to the class-II aminoacyl-tRNA synthetase family. Type-1 seryl-tRNA synthetase subfamily.
  • ドメインConsists of two distinct domains, a catalytic core and a N-terminal extension that is involved in tRNA binding.
  • 翻訳後修飾Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 細胞内局在Cytoplasm.
  • Information by UniProt
  • 参照データベース
  • 別名
    • C78314 antibody
    • cytoplasmic antibody
    • EC 6.1.1.11 antibody
    • FLJ36399 antibody
    • sarS antibody
    • Sars1 antibody
    • Serine tRNA ligase 1, cytoplasmic antibody
    • Serine tRNA ligase antibody
    • Serine--tRNA ligase antibody
    • serine--tRNA ligase, cytoplasmic antibody
    • SerRS antibody
    • SERS antibody
    • Seryl tRNA Ser/Sec synthetase antibody
    • Seryl tRNA synthetase antibody
    • Seryl tRNA synthetase cytoplasmic antibody
    • Seryl-tRNA Ser/Sec synthetase antibody
    • Seryl-tRNA synthetase antibody
    • seryl-tRNA synthetase, cytoplasmic antibody
    • Seryl-tRNA(Ser/Sec) synthetase antibody
    • Strs antibody
    • SYSC_HUMAN antibody
    see all

Anti-Seryl-tRNA synthetase antibody 画像

  • All lanes : Anti-Seryl-tRNA synthetase antibody (ab50146) at 1/1000 dilution

    Lane 1 : Whole cell lysate from rat hippocampal neurons (untreated)
    Lane 2 : Whole cell lysate from rat hippocampal neurons (treated with neurotrophin)
    Lane 3 : Whole cell lysate from rat hippocampal neurons (treated with inhibitor of the neurotrophin pathway 1 + neurotrophin)
    Lane 4 : Whole cell lysate from rat hippocampal neurons (treated with inhibitor of the neurotrophin pathway 2 + neurotrophin)
    Lane 5 : Whole cell lysate from rat hippocampal neurons (treated with inhibitor of the neurotrophin pathway 1)
    Lane 6 : Whole cell lysate from rat hippocampal neurons (treated with inhibitor of the neurotrophin pathway 2)

    Lysates/proteins at 40 µg per lane.

    Secondary
    Alkaline Phosphatase-conjugeted goat anti-rabbit IgG at 1/20000 dilution

    Performed under reducing conditions.

    Predicted band size : 59 kDa


    Exposure time : 3 minutes

    This image is courtesy of an anonymous Abreview

    See Abreview

  • Anti-Seryl-tRNA synthetase antibody (ab50146) at 1/10000 dilution + whole cell lysate prepared from HEK293 cells at 50 µg/ml

    Secondary
    Goat anti-rabbit IgG (H+L) at 1/10000 dilution

    Predicted band size : 59 kDa
    Observed band size : 55 kDa (why is the actual band size different from the predicted?)

Anti-Seryl-tRNA synthetase antibody (ab50146) 使用論文

ab50146 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Sample Rat Cell lysate - whole cell (hippocampal neurons)
Loading amount 40 µg
Specification hippocampal neurons
Treatment Neurotrophic factor
Gel Running Conditions Reduced Denaturing (10% acrylamide)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
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投稿 Sep 02 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"