Anti-Serum Response Factor SRF (phospho S103) 抗体 (ab53130)

製品の概要

  • 製品名
    Anti-Serum Response Factor SRF (phospho S103) antibody
    Serum Response Factor SRF 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Serum Response Factor SRF (phospho S103)
  • 特異性
    ab53130 detects endogenous levels of Serum Response Factor SRF only when phosphorylated at serine 103. In mouse and rat the modification site is serine 99.
  • アプリケーション
    適用あり: WB, IHC-P, ELISA, EMSAmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    A synthetic phosphopeptide derived from human Serum Response Factor SRF around the phosphorylation site of serine 103 (S-L-SP-E-M).

  • ポジティブ・コントロール
    • WB: LOVO cell extracts IHC-P: Human lung carcinoma tissue

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab53130 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/300 - 1/1000. Detects a band of approximately 55 kDa (predicted molecular weight: 52 kDa).
IHC-P Use at an assay dependent concentration.
ELISA 1/5000.
EMSA Use at an assay dependent concentration.

ターゲット情報

  • 機能
    SRF is a transcription factor that binds to the serum response element (SRE), a short sequence of dyad symmetry located 300 bp to the 5' of the site of transcription initiation of some genes (such as FOS). Required for cardiac differentiation and maturation.
  • 配列類似性
    Contains 1 MADS-box domain.
  • 翻訳後修飾
    Phosphorylated by PRKDC.
  • 細胞内局在
    Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • c fos serum response element binding factor antibody
    • c fos serum response element binding transcription factor antibody
    • ELK3 antibody
    • ERP antibody
    • MCM 1 antibody
    • MCM1 antibody
    • OTTHUMP00000039820 antibody
    • SAP2 antibody
    • Serum response factor antibody
    • SRF antibody
    • SRF serum response factor c fos serum response element binding transcription factor antibody
    • SRF_HUMAN antibody
    see all

画像

  • All lanes : Anti-Serum Response Factor SRF (phospho S103) antibody (ab53130) at 1/300 dilution

    Lane 1 : LOVO cell extract treated with serum (20%, 15mins)
    Lane 2 : LOVO cell extract treated with serum (20%, 15mins) with immunising peptide


    Predicted band size : 52 kDa
    Observed band size : 55 kDa (why is the actual band size different from the predicted?)
  • ab53130 at 1/50 dilution staining Serum Response Factor SRF in human lung carcinoma by Immunohistochemistry, Paraffin embedded tissue, in the absence and presence of the immunising peptide.ab53130 at 1/50 dilution staining Serum Response Factor SRF in human lung carcinoma by Immunohistochemistry, Paraffin embedded tissue, in the absence and presence of the immunising peptide.

参考文献

This product has been referenced in:
  • Heller EA  et al. Locus-specific epigenetic remodeling controls addiction- and depression-related behaviors. Nat Neurosci 17:1720-7 (2014). Read more (PubMed: 25347353) »
  • Vissing K  et al. Effect of resistance exercise contraction mode and protein supplementation on members of the STARS signalling pathway. J Physiol 591:3749-63 (2013). Read more (PubMed: 23753523) »

See all 4 Publications for this product

レビューと Q&A

Thank you for contacting us. Unfortunately, this specific lysate (LOVO cell extract treated with serum (20%, 15min)) is not available for sale. Alternative positive controls would be a human lung carcinoma lysate (e.g. ab14870 or ab14871...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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