Anti-Serum Response Factor SRF 抗体 (ab91477)

製品の概要

  • 製品名
    Anti-Serum Response Factor SRF antibody
    Serum Response Factor SRF 一次抗体 製品一覧
  • 製品の詳細
    Goat polyclonal to Serum Response Factor SRF
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Cow, Dog
  • 免疫原

    Synthetic peptide:

    C-QASPSRDSSTDLTQ

    , corresponding to internal sequence amino acids 368-381 of Human Serum Response Factor SRF (NP_003122.1).

  • ポジティブ・コントロール
    • Human lung fibroblasts lysates.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab91477 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 67 kDa (predicted molecular weight: 52 kDa).
Approx 67kDa band observed in primary cultured human lung fibroblasts lysates after 24 h treatment with 1ng/ml TGF-bl (calculated MW of 51.6kDa according to NP_003122.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Davis FJ et al, Am J Physiol Heart Circ Physiol. 2002 Apr;282(4):H1521-33.; PMID: 11893590).

ターゲット情報

  • 機能
    SRF is a transcription factor that binds to the serum response element (SRE), a short sequence of dyad symmetry located 300 bp to the 5' of the site of transcription initiation of some genes (such as FOS). Required for cardiac differentiation and maturation.
  • 配列類似性
    Contains 1 MADS-box domain.
  • 翻訳後修飾
    Phosphorylated by PRKDC.
  • 細胞内局在
    Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • c fos serum response element binding factor antibody
    • c fos serum response element binding transcription factor antibody
    • ELK3 antibody
    • ERP antibody
    • MCM 1 antibody
    • MCM1 antibody
    • OTTHUMP00000039820 antibody
    • SAP2 antibody
    • Serum response factor antibody
    • SRF antibody
    • SRF serum response factor c fos serum response element binding transcription factor antibody
    • SRF_HUMAN antibody
    see all

画像

  • All lanes : Anti-Serum Response Factor SRF antibody (ab91477) at 1 µg/ml

    Lane 1 : Un-treated primary cultured human lung fibroblast lysate.
    Lane 2 : TGF-bl-treated primary cultured human lung fibroblast lysate (24hrs, 1ng/ml).

    Developed using the ECL technique

    Predicted band size : 52 kDa
    Observed band size : 67 kDa (why is the actual band size different from the predicted?)


    Exposure time : 1 hour

プロトコール

参考文献

ab91477 has not yet been referenced specifically in any publications.

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