Anti-Serum Response Factor SRF 抗体 (ab101246)

製品の概要

  • 製品名
    Anti-Serum Response Factor SRF antibody
    Serum Response Factor SRF 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Serum Response Factor SRF
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Horse, Chicken, Cow, Dog, Pig
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 200 - 300 of Human Serum Response Factor SRF.

  • ポジティブ・コントロール
    • This antibody gave a positive signal in the following whole cell lysates: HeLa; HeLa Nuclear; Jurkat; HEK293; MCF7; NIH3T3; F9.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab101246 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 51 kDa (predicted molecular weight: 51 kDa).

ターゲット情報

  • 機能
    SRF is a transcription factor that binds to the serum response element (SRE), a short sequence of dyad symmetry located 300 bp to the 5' of the site of transcription initiation of some genes (such as FOS). Required for cardiac differentiation and maturation.
  • 配列類似性
    Contains 1 MADS-box domain.
  • 翻訳後修飾
    Phosphorylated by PRKDC.
  • 細胞内局在
    Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • c fos serum response element binding factor antibody
    • c fos serum response element binding transcription factor antibody
    • ELK3 antibody
    • ERP antibody
    • MCM 1 antibody
    • MCM1 antibody
    • OTTHUMP00000039820 antibody
    • SAP2 antibody
    • Serum response factor antibody
    • SRF antibody
    • SRF serum response factor c fos serum response element binding transcription factor antibody
    • SRF_HUMAN antibody
    see all

画像

  • All lanes : Anti-Serum Response Factor SRF antibody (ab101246) at 1 µg

    Lane 1 : : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate
    Lane 3 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 4 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
    Lane 5 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
    Lane 6 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
    Lane 7 : F9 (Mouse embryonic carcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 51 kDa
    Observed band size : 51 kDa
    Additional bands at : 100 kDa,40 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 20 minutes

プロトコール

参考文献

ab101246 has not yet been referenced specifically in any publications.

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