製品の概要

  • 製品名Anti-Serum Amyloid P antibody [6E6]
    Serum Amyloid P 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [6E6] to Serum Amyloid P
  • アプリケーション適用あり: WB, IP, ELISAmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Full length native protein purified from Human plasma

  • ポジティブ・コントロール
    • Human plasma

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab51085 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000. Predicted molecular weight: 25 kDa.
IP Use at an assay dependent dilution.
ELISA Use at an assay dependent dilution.

ターゲット情報

  • 機能Can interact with DNA and histones and may scavenge nuclear material released from damaged circulating cells. May also function as a calcium-dependent lectin.
  • 組織特異性Found in serum and urine.
  • 関連疾患Note=SAP is a precursor of amyloid component P which is found in basement membrane and associated with amyloid deposits.
  • 配列類似性Belongs to the pentaxin family.
    Contains 1 pentaxin domain.
  • 翻訳後修飾N-glycosylated with a complex biantennary oligosaccharide chain with a sialic acid at the end (disialo-SAP). Monosialo-SAP as well as asioalo-SAP are also detected (PubMed:15174148).
  • 細胞内局在Secreted.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 9.5S alpha 1 glycoprotein antibody
    • 9.5S alpha-1-glycoprotein antibody
    • Amyloid P component serum antibody
    • APCS antibody
    • MGC88159 antibody
    • Pentaxin related antibody
    • Pentraxin 2 antibody
    • PTX 2 antibody
    • PTX2 antibody
    • SAMP_HUMAN antibody
    • SAP antibody
    • Serum amyloid P component antibody
    • Serum amyloid P-component(1-203) antibody
    see all

Anti-Serum Amyloid P antibody [6E6] 画像

  • All lanes : Anti-Serum Amyloid P antibody [6E6] (ab51085) at 1/1000 dilution

    Lane 1 : Amyloid P protein isolated from human plasma
    Lane 2 : Human plasma


    Predicted band size : 25 kDa
    Observed band size : 28 kDa (why is the actual band size different from the predicted?)

Anti-Serum Amyloid P antibody [6E6] (ab51085) 使用論文

This product has been referenced in:
  • Gu BJ  et al. P2X7 receptor-mediated scavenger activity of mononuclear phagocytes toward non-opsonized particles and apoptotic cells is inhibited by serum glycoproteins but remains active in cerebrospinal fluid. J Biol Chem 287:17318-30 (2012). WB ; Human . Read more (PubMed: 22461619) »

See 1 Publication for this product

Product Wall

Thank you for the clarification about the blocking buffer. BSA should be fine, but if you are seeing no signal at all, you could try reducing the percentage of BSA 10-fold, to 0.5%. This might allow a signal to develop more easily.

Human ser...

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Your protocol looks mostly correct but my one concern is the antibody diluent, 5% FBS. Are you confident the Fetal Bovine Serum does not contain the target of the antibody, serum amyloid P? I think it is likely that the antibody is reacting with this i...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"