製品の概要

  • 製品名Anti-SerpinB3 antibody
    SerpinB3 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal to SerpinB3
  • アプリケーション適用あり: WB, Flow Cyt, ICC/IF, IHC-Pmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Recombinant fragment, corresponding to amino acids 276-391 of Human SerpinB3

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファーPreservative: None
    PBS, pH 7.2
  • Concentration information loading...
  • 精製度Protein G purified
  • ポリ/モノモノクローナル
  • アイソタイプIgG2a
  • 軽鎖の種類kappa
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab55733 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 - 5 µg/ml. This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.
Flow Cyt Use 0.1µg for 106 cells. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
ICC/IF Use at an assay dependent concentration. PubMed: 23185467
IHC-P Use at an assay dependent concentration. PubMed: 23185467

ターゲット情報

  • 機能May act as a protease inhibitor to modulate the host immune response against tumor cells.
  • 組織特異性Squamous cells. Expressed in some hepatocellular carcinoma (at protein level).
  • 配列類似性Belongs to the serpin family. Ov-serpin subfamily.
  • 発生段階Its expression is closely related to cellular differentiation in both normal and malignant squamous cells.
  • 細胞内局在Cytoplasm. Seems to also be secreted in plasma by cancerous cells but at a low level.
  • Information by UniProt
  • 参照データベース
  • 別名
    • HsT1196 antibody
    • Protein T4 A antibody
    • Protein T4-A antibody
    • SCC antibody
    • SCCA antibody
    • SCCA PD antibody
    • SCCA-1 antibody
    • SCCA-PD antibody
    • SCCA1 antibody
    • SCCAPD antibody
    • Serine (or cysteine) proteinase inhibitor clade B (ovalbumin) member 3 antibody
    • Serpin B3 antibody
    • Serpin peptidase inhibitor clade B (ovalbumin) member 3 antibody
    • serpin peptidase inhibitor, clade B (ovalbumin), member 3 antibody
    • SERPINB3 antibody
    • SPB3_HUMAN antibody
    • Squamous cell carcinoma antigen 1 antibody
    • T4 A antibody
    • T4-A antibody
    • T4A antibody
    see all

Anti-SerpinB3 antibody 画像

  • Overlay histogram showing A431 cells stained with ab55733 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab55733, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
  • Western blot against tagged recombinant protein immunogen using ab55733 SerpinB3 antibody at 1ug/ml. Predicted band size of immunogen is 39 kDa

Anti-SerpinB3 antibody (ab55733) 使用論文

This product has been referenced in:
  • Lim W  et al. SERPINB3 in the Chicken Model of Ovarian Cancer: A Prognostic Factor for Platinum Resistance and Survival in Patients with Epithelial Ovarian Cancer. PLoS One 7:e49869 (2012). ICC/IF, IHC-P ; Human, Chicken . Read more (PubMed: 23185467) »
  • Burgener A  et al. Identification of differentially expressed proteins in the cervical mucosa of HIV-1-resistant sex workers. J Proteome Res 7:4446-54 (2008). WB ; Human . Read more (PubMed: 18707157) »

See all 2 Publications for this product

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I can confirm that although we have not tested these antibodies for cross-reactivity with Serpin B4 but there is high percentage of immunogen sequence homology. It means the chances of cross reactivity are high. Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"