Anti-Serotonin N-acetyltransferase 抗体 (ab3505)


  • 製品名Anti-Serotonin N-acetyltransferase antibody
    Serotonin N-acetyltransferase 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Serotonin N-acetyltransferase
  • アプリケーション適用あり: WB, IHC-Pmore details
  • 種交差性
    交差種: Rat
    交差が予測される動物種: Mouse, Chicken, Hamster, Human, Chimpanzee, Rhesus monkey
  • 免疫原

    Synthetic peptide corresponding to Mouse Serotonin N-acetyltransferase aa 24-33 (N terminal).


    (Peptide available as ab5003)

  • ポジティブ・コントロール
    • Rat pineal gland whole protein extract IHC-P: FFPE Rat brain tissue sections.
  • 特記事項



  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファーPreservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 99% PBS
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • 特記事項(精製)Total anti-N-terminal AA-NAT-specific IgG was affinity purified on resin-coupled non-phosphopeptide. Phospho-specific amino-terminal IgG was subsequently isolated on resin-coupled phosphopeptide.
  • 一次抗体 備考Pineal gland serotonin N-acetyltransferase (arylalkylamine N-acetyltransferase, AA-NAT) is an ~23 kDa protein which is the rate-limiting enzyme in melatonin synthesis. Melatonin serves as the hormonal signal of the daily light/dark cycle, or body clock. The nocturnal oscillation in enzyme activity ranges by 10- to 100-fold depending on species among vertebrates. Studies have shown that AA-NAT is regulated by cAMP at both the mRNA and protein levels. AA-NAT has putative protein kinase A (PKA) sites at Thr 29 and Ser 206. Phosphorylation of AA-NAT at these residues might play a role in protein inactivation via proteasome-dependent degradation.
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野



Our Abpromise guarantee covers the use of ab3505 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 0.5 µg/ml. Detects a band of approximately 23 kDa (predicted molecular weight: 23 kDa).Can be blocked with Serotonin N-acetyltransferase peptide (ab5003).

This antibody detects an ~23 kDa protein representing unphosphorylated and phosphorylated AA-NAT from rat pineal gland whole protein extract.

IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.


  • 機能Controls the night/day rhythm of melatonin production in the pineal gland. Catalyzes the N-acetylation of serotonin into N-acetylserotonin, the penultimate step in the synthesis of melatonin.
  • 組織特異性Highly expressed in pineal gland and at lower levels in the retina. Weak expression in several brain regions and in the pituitary gland.
  • パスウェイAromatic compound metabolism; melatonin biosynthesis; melatonin from serotonin: step 1/2.
  • 関連疾患Delayed sleep phase syndrome
  • 配列類似性Belongs to the acetyltransferase family. AANAT subfamily.
    Contains 1 N-acetyltransferase domain.
  • 翻訳後修飾cAMP-dependent phosphorylation on both N-terminal Thr-31 and C-terminal Ser-205 regulates AANAT activity by promoting interaction with 14-3-3 proteins.
  • 細胞内局在Cytoplasm.
  • Information by UniProt
  • 参照データベース
  • 別名
    • AA-NAT antibody
    • Aanat antibody
    • Aralkylamine N-acetyltransferase antibody
    • Arylalkylamine N acetyltransferase antibody
    • Serotonin acetylase antibody
    • Serotonin N-acetyltransferase antibody
    • SNAT antibody
    • SNAT_HUMAN antibody
    see all

Anti-Serotonin N-acetyltransferase antibody 画像

  • IHC image of Serotonin N-acetyltransferase staining in Rat brain formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab3505, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-Serotonin N-acetyltransferase antibody (ab3505) 使用論文

ab3505 has not yet been referenced specifically in any publications.

Product Wall

Regarding the feedback on the secondary and the loading control working well, this is very reassuring and tells us the problem is not due to these. As the customer is sure the cells she is investigating are still pineal and should definitely express Se...

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