Anti-Serotonin 抗体 (ab6444)

製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to Serotonin
  • 特異性
    Using a conjugate 5-Hydroxytryptamine-(protein carriers), antibody specificity was performed with an ELISA test by competition experiments with the following compounds
    CompoundCross-reactivity ratio (a)
    Serotonin-G-(Pc)1
    5-Methoxytryptamine-G-(Pc)1/25
    Tryptamine-G-(Pc)1/50
    5-Hydroxytryptophan-G-(Pc)1/175
    L-Tryptophan-G-(Pc)1/3500
    5-Methoxytryptophan-G-(Pc)1/20000
    free Serotonin 1/115
    (a) : 5-Hydroxytryptamine-G-(Pc) concentration / Other c conjugated indolealkylamines concentration at half displacement ; G = Glutaraldehyde
  • アプリケーション
    適用あり: ELISA, ICCmore details
  • 免疫原

    Chemical/ Small Molecule corresponding to Serotonin. Synthetic 5-Hydroxytryptamine conjugated to protein carrier (PC)

  • 特記事項

    ab6444 specific to conjugated 5-Hydroxytryptamine (Serotonin)

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Concentration information loading...
  • 精製度
    Ammonium Sulphate Precipitation
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab6444 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA Use at an assay dependent concentration.
ICC Use at an assay dependent concentration.

ターゲット情報

  • 関連性
    Serotonin (5-hydroxytryptamine, or 5-HT) is a monoamine neurotransmitter synthesised in serotonergic neurons in the central nervous system and enterochromaffin cells in the gastrointestinal tract. Serotonin plays an important part in the biochemistry of depression, migraine, bipolar disorder and anxiety. It is also believed to be influential on sexuality and appetite. 5-HT is generally thought not to be released from synaptic terminal buttons in the manner of classical neurotransmission but from serotonergic varacosities into the extra neuronal space. From here it is free to diffuse over a relatively large region of space (>20µm) and activate 5-HT receptors located on the dendrites, cell bodies and presynaptic terminals of adjacent neurons. Serotonergic action is terminated primarily via uptake of 5-HT from the synapse. This is through the specific monoamine transporter for 5-HT, 5-HT reuptake transporter, on the presynaptic neuron. The pharmacology of 5-HT is extremely complex, with its actions being mediated by a large and diverse range of 5-HT receptors.
  • 細胞内局在
    Cytoplasmic and Secreted
  • 別名
    • 5 hydroxytryptamine antibody
    • 5HT antibody

プロトコール

Anti-Serotonin antibody (ab6444) 使用論文

ab6444 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Colon)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate buffer pH6.0
Permeabilization
No
Specification
Colon
Blocking step
hydrogen peroxide as blocking agent for 15 minute(s) · Concentration: 3% · Temperature: 24°C
Fixative
Formaldehyde
Username

Vinicius Kannen Cardoso

Verified customer

投稿 Aug 10 2016

Thank you for your call last week and for your patience while I have been in touch with the lab regarding your enquiry.

There is a mistake on the datasheet, and the antibody was raised against serotonin conjugated to a protein carrier atthe a...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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