Anti-Serine/threonine-protein kinase 4 抗体 (ab57836)




Our Abpromise guarantee covers the use of ab57836 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 56 kDa.
ICC/IF Use a concentration of 10 µg/ml.
IHC-P Use a concentration of 5 µg/ml.
Flow Cyt Use 2µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.


  • 機能Stress-activated, pro-apoptotic kinase which, following caspase-cleavage, enters the nucleus and induces chromatin condensation followed by internucleosomal DNA fragmentation. Key component of the Hippo signaling pathway which plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein MST1/MST2, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Phosphorylation of YAP1 by LATS2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. MST1/MST2 are required to repress proliferation of mature hepatocytes, to prevent activation of facultative adult liver stem cells (oval cells), and to inhibit tumor formation (By similarity). Phosphorylates 'Ser-14' of histone H2B (H2BS14ph) during apoptosis. Phosphorylates FOXO3 upon oxidative stress, which results in its nuclear translocation and cell death initiation.
  • 組織特異性Ubiquitously expressed.
  • 配列類似性Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
    Contains 1 protein kinase domain.
    Contains 1 SARAH domain.
  • 翻訳後修飾Autophosphorylated on serine and threonine residues.
  • 細胞内局在Cytoplasm. Nucleus. The caspase-cleaved form cycles between the nucleus and cytoplasm.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Kinase responsive to stress antibody
    • Krs2 antibody
    • Mammalian STE20 like protein kinase 1 antibody
    • Mammalian STE20-like protein kinase 1 antibody
    • Mammalian sterile 20 like 1 antibody
    • MST-1 antibody
    • MST1 antibody
    • Serine/threonine kinase 4 antibody
    • Serine/threonine protein kinase Krs 2 antibody
    • Serine/threonine-protein kinase 4 antibody
    • Serine/threonine-protein kinase Krs-2 antibody
    • STE20 like kinase MST1 antibody
    • STE20-like kinase MST1 antibody
    • STK4 antibody
    • STK4_HUMAN antibody
    • TIIAC antibody
    • YSK3 antibody
    see all

Anti-Serine/threonine-protein kinase 4 antibody 画像

  •  Serine/threonine-protein kinase 4antibody (ab57836) used in immunofluorescence at 10ug/ml on HeLa cells.

  •  Serine/threonine-protein kinase 4antibody (ab57836) used in immunohistochemistry at 5ug/ml on formalin fixed and paraffin embedded human stomach carcinoma tissue.

  • Predicted band size : 56 kDa
    MST1 antibody (ab57836) at 1ug/lane + HeLa cell lysate at 25ug/lane.
  • Overlay histogram showing HeLa cells stained with ab57836 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab57836, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

Anti-Serine/threonine-protein kinase 4 antibody (ab57836) 使用論文

This product has been referenced in:
  • Serrano I  et al. Inactivation of the Hippo tumour suppressor pathway by integrin-linked kinase. Nat Commun 4:2976 (2013). WB ; Human . Read more (PubMed: 24356468) »
  • Goldie SJ  et al. FRMD4A upregulation in human squamous cell carcinoma promotes tumor growth and metastasis and is associated with poor prognosis. Cancer Res 72:3424-36 (2012). Human . Read more (PubMed: 22564525) »

See all 2 Publications for this product

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As you have observed on the datasheet of the anti-MST1 antibody (ab57836), we have observed the band at an expected size of ˜55 kDa, as well as a band of ˜37 kDa when performing W...

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