This antibody gave a positive signal in the following tissue lysates within Western blot: Human Brain; Mouse Brain: Rat Brain; Mouse Hippocampus; Rat Hippocampus.
This antibody also gave a positive signal in SHSY-5Y whole cell lysate within Immunofluorescence.
保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml.
Use a concentration of 5 µg/ml.
機能Catalyzes the synthesis of D-serine from L-serine. D-serine is a key coagonist with glutamate at NMDA receptors. Has dehydratase activity towards both L-serine and D-serine.
組織特異性Brain: expressed at high levels in hippocampus and corpus callosum, intermediate levels in substantia nigra and caudate, and low levels in amygdala, thalamus, and subthalamic nuclei. Expressed in heart, skeletal muscle, kidney and liver.
配列類似性Belongs to the serine/threonine dehydratase family.
翻訳後修飾S-nitrosylated, leading to decrease the enzyme activity.
ICC/IF image of ab45545 stained human SHSY5Y cells. The cells were methanol fixed (10 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab45545, 5µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
Western blot - Serine racemase antibody (ab45545)
All lanes : Anti-Serine racemase antibody (ab45545) at 1 µg/ml
Lane 1 : Human brain tissue lysate - total protein (ab29466) Lane 2 : Brain (Rat) Tissue Lysate Lane 3 : Brain (Mouse) Tissue Lysate Lane 4 : Hippocampus (Rat) Tissue Lysate Lane 5 : Hippocampus (Mouse) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution