製品の概要

  • 製品名Anti-Serine racemase antibody
    Serine racemase 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Serine racemase
  • アプリケーション適用あり: WB, ICC/IFmore details
  • 種交差性
    交差種: Mouse
    交差が予測される動物種: Human
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Human Serine racemase.

    (Peptide available as ab45544.)

  • ポジティブ・コントロール
    • This antibody gave a positive signal in the following tissue lysates within Western blot: Human Brain; Mouse Brain: Rat Brain; Mouse Hippocampus; Rat Hippocampus. This antibody also gave a positive signal in SHSY-5Y whole cell lysate within Immunofluorescence.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab45545 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml.
ICC/IF Use a concentration of 5 µg/ml.

ターゲット情報

  • 機能Catalyzes the synthesis of D-serine from L-serine. D-serine is a key coagonist with glutamate at NMDA receptors. Has dehydratase activity towards both L-serine and D-serine.
  • 組織特異性Brain: expressed at high levels in hippocampus and corpus callosum, intermediate levels in substantia nigra and caudate, and low levels in amygdala, thalamus, and subthalamic nuclei. Expressed in heart, skeletal muscle, kidney and liver.
  • 配列類似性Belongs to the serine/threonine dehydratase family.
  • 翻訳後修飾S-nitrosylated, leading to decrease the enzyme activity.
  • Information by UniProt
  • 参照データベース
  • 別名
    • D serine ammonia lyase antibody
    • D serine dehydratase antibody
    • D-serine ammonia-lyase antibody
    • D-serine dehydratase antibody
    • ILV1 antibody
    • ISO1 antibody
    • L serine ammonia lyase antibody
    • L serine dehydratase antibody
    • L-serine ammonia-lyase antibody
    • L-serine dehydratase antibody
    • Serine racemase antibody
    • srr antibody
    • SRR_HUMAN antibody
    see all

Anti-Serine racemase antibody 画像

  • ICC/IF image of ab45545 stained human SHSY5Y cells. The cells were methanol fixed (10 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab45545, 5µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue). 

  • All lanes : Anti-Serine racemase antibody (ab45545) at 1 µg/ml

    Lane 1 : Human brain tissue lysate - total protein (ab29466)
    Lane 2 : Brain (Rat) Tissue Lysate
    Lane 3 : Brain (Mouse) Tissue Lysate
    Lane 4 : Hippocampus (Rat) Tissue Lysate
    Lane 5 : Hippocampus (Mouse) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Observed band size : 37 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 100 kDa,60 kDa,70 kDa. We are unsure as to the identity of these extra bands.

Anti-Serine racemase antibody (ab45545) 使用論文

This product has been referenced in:
  • Wang W & Barger SW Cross-linking of serine racemase dimer by reactive oxygen species and reactive nitrogen species. J Neurosci Res 90:1218-29 (2012). WB ; Mouse . Read more (PubMed: 22354542) »

See 1 Publication for this product

Product Wall

Application Western blot
Sample Mouse Cell lysate - whole cell (HAPI mouse microglia cell line)
Loading amount 50 µg
Specification HAPI mouse microglia cell line
Treatment 30 ng/ml LPS for 16 hours
Gel Running Conditions Reduced Denaturing (10%)
Blocking step I-Block as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.2% · Temperature: 22°C
Username

Abcam user community

Verified customer

投稿 Apr 11 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"