製品の概要

  • 製品名Anti-SERCA1 ATPase antibody
    SERCA1 ATPase 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to SERCA1 ATPase
  • アプリケーション適用あり: ICC/IF, WB, ELISAmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Rat
  • 免疫原

    Synthesized peptide corresponding to an internal sequence of Human SERCA1 ATPase.

  • ポジティブ・コントロール
    • NIH 3T3, A549 or HT29 cell lysate. IF/ICC: HeLa cell line

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab92666 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF
WB
ELISA
  • 追加情報ELISA: 1/10,000.
    WB: 1/500 - 1/1000. Predicted molecular weight: 110 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能Key regulator of striated muscle performance by acting as the major Ca(2+) ATPase responsible for the reuptake of cytosolic Ca(2+) into the sarcoplasmic reticulum. Catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen. Contributes to calcium sequestration involved in muscular excitation/contraction.
    • 組織特異性Skeletal muscle, fast twitch muscle (type II) fibers.
    • 関連疾患Brody myopathy
    • 配列類似性Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIA subfamily.
    • 発生段階Isoform SERCA1A accounts for more than 99% of SERCA1 isoforms expressed in adult skeletal muscle, while isoform SERCA1B predominates in neo-natal skeletal muscle.
    • 細胞内局在Endoplasmic reticulum membrane. Sarcoplasmic reticulum membrane.
    • Information by UniProt
    • 参照データベース
    • 別名
      • fast twitch skeletal muscle isoform antibody
      • AT2A1_HUMAN antibody
      • ATP2A antibody
      • ATP2A1 antibody
      • ATPase Ca++ transporting cardiac muscle fast twitch 1 antibody
      • ATPase Ca++ transporting fast twitch 1 antibody
      • ATPase, Ca(2+)-transporting fast twitch 1 antibody
      • Calcium pump 1 antibody
      • Calcium transporting ATPase sarcoplasmic reticulum type fast twitch skeletal muscle isoform antibody
      • Calcium-transporting ATPase sarcoplasmic reticulum type antibody
      • EC 3.6.3.8 antibody
      • Endoplasmic reticulum class 1/2 Ca(2+) ATPase antibody
      • Fast skeletal muscle SR calcium ATPase antibody
      • OTTHUMP00000162561 antibody
      • OTTHUMP00000162562 antibody
      • Sarcoendoplasmic reticulum calcium ATPase antibody
      • Sarcoplasmic reticulum Ca(2+)-ATPase 1 antibody
      • Sarcoplasmic/endoplasmic reticulum calcium ATPase 1 antibody
      • SERCA 1 antibody
      • SERCA1 antibody
      • SERCA1 truncated isoform, included antibody
      • SR Ca(2+) ATPase 1 antibody
      • SR Ca(2+)-ATPase 1 antibody
      see all

    Anti-SERCA1 ATPase antibody 画像

    • ICC/IF image of ab92666 stained HeLa cells. The cells were 100% Methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab92666, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    • All lanes : Anti-SERCA1 ATPase antibody (ab92666) at 1/500 dilution

      Lane 1 : NIH3T3 cell lysate
      Lane 2 : A549 cell lysate
      Lane 3 : HT29 cell lysate
      Lane 4 : A549 cell lysate with immunizing peptide at 10 µg

      Lysates/proteins at 30 µg per lane.


      Predicted band size : 110 kDa

    Anti-SERCA1 ATPase antibody (ab92666) 使用論文

    ab92666 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"